Abstract：Objective: To investigate the effect of connective tissue growth factor (CTGF) on the proliferation in human pancreatic cancer cell line SW1990, with a focus on the expression of matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor (VEGF). This study was designed to explore the underlying mechanism for the role of CTGF in the development of pancreatic cancer. Methods: pcDNA3.0CTGF and pcDNA3.0 were transfected into SW1990 cells through lipofectamine and positive clones were screened by G418. RT-PCR and Western blotting were employed to identify mRNA and protein expression of CTGF in SW1990 cells, respectively. The expression of MMP-9 and VEGF in positive clones was detected by RT-PCR and Western blotting.Cell viability was assessed by dimethylthiazol diphenyl tetrazoliumbromide (MTT) method. Results: Positive clones C-5 and C-28 with CTGF over-expression were successfully established. Compared with non- transfected control group, the expressions of MMP-9 and VEGF in C-5 and C-28 was increased significantly, and the proliferation level of C-5 and C-28 was increased significantly. Conclusions: CTGF transfection could effectively enhance the expression of MMP-9 and VEGF and the proliferation in SW1990 cells which suggested a potential role for CTGF in gene therapy of pancreatic cancer.
姜建国1， 焦霞2*， 朱晓蔚2， 戴桂红2， 肖蔚2， 刘欣韵2， 窦荣荣2， 周彤敏2， 于鸿2. CTGF基因转染对胰腺癌细胞MMP9、VEGF表达及增殖的影响[J]. 江苏大学学报:医学版, 2013, 23(5): 377-381.
JIANG Jian-guo1, JIAO Xia2, ZHU Xiao-wei2. Effect of gene CTGF transfection on the expression of MMP-9 and VEGF and proliferation in human pancreatic cancer cells. Journal of Jiangsu University(Medicine Edition), 2013, 23(5): 377-381.
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