Abstract:Objective: To investigate the effect of connective tissue growth factor (CTGF) on the proliferation in human pancreatic cancer cell line SW1990, with a focus on the expression of matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor (VEGF). This study was designed to explore the underlying mechanism for the role of CTGF in the development of pancreatic cancer. Methods: pcDNA3.0CTGF and pcDNA3.0 were transfected into SW1990 cells through lipofectamine and positive clones were screened by G418. RT-PCR and Western blotting were employed to identify mRNA and protein expression of CTGF in SW1990 cells, respectively. The expression of MMP-9 and VEGF in positive clones was detected by RT-PCR and Western blotting.Cell viability was assessed by dimethylthiazol diphenyl tetrazoliumbromide (MTT) method. Results: Positive clones C-5 and C-28 with CTGF over-expression were successfully established. Compared with non- transfected control group, the expressions of MMP-9 and VEGF in C-5 and C-28 was increased significantly, and the proliferation level of C-5 and C-28 was increased significantly. Conclusions: CTGF transfection could effectively enhance the expression of MMP-9 and VEGF and the proliferation in SW1990 cells which suggested a potential role for CTGF in gene therapy of pancreatic cancer.
姜建国1, 焦霞2*, 朱晓蔚2, 戴桂红2, 肖蔚2, 刘欣韵2, 窦荣荣2, 周彤敏2, 于鸿2. CTGF基因转染对胰腺癌细胞MMP9、VEGF表达及增殖的影响[J]. 江苏大学学报:医学版, 2013, 23(5): 377-381.
JIANG Jian-guo1, JIAO Xia2, ZHU Xiao-wei2. Effect of gene CTGF transfection on the expression of MMP-9 and VEGF and proliferation in human pancreatic cancer cells. Journal of Jiangsu University(Medicine Edition), 2013, 23(5): 377-381.
[1]Phanish MK, Winn SK, Dockrell ME. Connective tissue growth factor-(CTGF, CCN2):a marker, mediator and therapeutic target for renal fibrosis[J].Nephron Exp Nephrol,2010,114(3):e83-92.[2]Dhar A, Ray A. The CCN family proteins in carcinogenesis[J].Exp Oncol,2010,32(1):2-9.[3]许斌,陈寅,戴桂红,等.转染CTGF基因对乳腺癌细胞MMP-2及TIMP-2表达的影响[J].江苏大学学报:医学版,2012,22(2):97-100.[4]钱华,王华,袁冬兰,等. 转染NDRG1基因对宫颈癌细胞COX-2、VEGF表达及增殖的影响[J]. 江苏大学学报:医学版,2012,22(6):472-475.[5]Yu H, Zhao G, LI H, et al. Candesartan antagonizes pressure overload-evoked cardiac remodeling through Smad7 genedependent MMP-9 suppression[J]. Gene,2012, 497(2): 301-306.[6]Bennewith KL, Huang X, Ham CM, et al. The role of tumor cell-derived connective tissue growth factor(CTGF/CCN2) in pancreatic tumor growth[J].Cancer Res,2009,69(3): 775-784.[7]Chen PS, Wang MY, Wu SN, et al. CTGF enhances the motility of breast cancer cells via an integrin-alphavbeta3-ERK1/2-dependent S100A4-upregulated pathway[J].J Cell Sci,2007,120(12):2053-2065.[8]Gondi CS, Rao JS. Therapeutic potential of siRNA-mediated targeting of urokinase plasminogen activator, its receptor, and matrix metalloproteinases[J]. Methods Mol Biol,2009,487:267-281.[9]Yang F, Jin C, Jiang YJ, et al. Potential role of soluble VEGFR-1 in antiangiogenesis therapy for cancer[J]. Expert Rev Anticancer Ther,2011,11(4):541-549.[10]Wang GB, Zhou XY, Yuan T, et al. Significance of serum connective tissue growth factor in patients with hepatocellular carcinoma and relationship with angiogenesis[J]. World J Surg,2010,34(10):2411-2417.[11]Gao YB, Xiang ZL, Zhou LY, et al. Enhanced production of CTGF and IL-11 from highly metastatic hepatoma cells under hypoxic conditions :an implication of hepatocellular carcinoma metastasis to bone[J]. J Cancer Res Clin Oncol,2013,139(4):669-679.[12]Pickles M, Leask A. Analysis of CCN2 promoter activity in PANC-1 cells:regulation by ras/MEK/ERK[J].J Cell Commun Signal,2007,1(2):85-90.[13]Kwon S, Munroe X, Crawley SC, et al. Expression of connective tissue growth factor in pancreatic cancer cell lines[J]. Int J Oncol,2007,31(4):693-703.