Characteristics and clinical relevance of Helicobacter pylorivacA i、d alleles in Zhenjiang patients with gastrointestinal disease
JIANG Shuang-hong1, WU Ying1, HE Ming-jie1, YU Xiao-yan1, WANG Jing1, JIANG Xiao-meng1，ZHOU Hong2
（1.Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001； 2.School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang Jiangsu 212001, China）
Abstract：Objective：To study the characteristics and clinical relevance of Helicobacter pylori (H.pylori) two major virulence markers: the vacuolation toxin(vacA) i、d alleles and the cytotoxin-associated gene product(cagA) status in Zhenjiang patients with gastrointestinal disease. Methods: Rapid urease test and/or histology was performed to diagnose the gastric antrum mucosa of H.pylori infection. The vacA s-, m-, i-and d-region genotypes and cagA status were investigated by polymerase chain reaction(PCR). Results: In 179 H.pylori cases, 2 was mixed infections(1.1%，2/179). The genotypes of vacA were found: i1(96.6%, 171/177), i2(1.1%, 2/177) and d1(98.9%, 175/177), d2(1.1%, 2/177)；The positive rates of cagA gene was 97.7%(173/177), the major recombination was s1/m2/i1/d1(56.5%), followed by s1/m1/i1/d1(38.4%). VacA s2/m2 was associated with i2, d2(P<0.05). s1, i1, s1/i1 and cagA positive status was associated with d1(both P<0.05).The distribution of s, m, i, d alleles and cagA status showed no significant difference among chronic gastritis, peptic ulcer and gastric cancer(both P＞0.05). Conclusion: s1/m2/i1/d1/cagA(+) of H.pylori was the major recombination in Zhenjiang patients with gastrointestinal disease. There was a correlation among H.pylori genotypes. There was no association between vacA genotypes, cagA status and the distribution of gastrointestinal diseases.
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2013, Vol. 23 
