Abstract：Objective: To observe the effects of MTA1 small interfering RNA（siRNA）on anchorage-independent growth and anoikis of bladder cancer cell line BIU-87. Methods: After construction of the MTA1 gene eukaryotic expression vector of siRNA and transfected to the bladder cancer cell line BIU-87, the expression of MTA1 gene was detected by real-time PCR and western blot; to detect anchorage-independent growth using clon formation in soft agar,and anoikis using DNA fragmentation assay and flow cytometry assay，respectively. Results: The level of mRNA and protein of MTA1 of groups transfectd with siRNA were decreased markedly in a time-and dose-dependent manner. MTA1 siRNA can inhibit anchorage-independent growth in a dose-dependent manner. MTA1 can reduced the resistance to anoikis of BIU-87 cells in a dose-and time-dependent manner. Conclusion: MTA1 siRNA can inhibit the growth of bladder cancer cell line BIU87 through inducing anoikis.
引用本文:
崔飞伦1, 2 , 邱镇1, 胡建鹏1, 范钰2. RNA干扰沉默MTA1基因对膀胱癌细胞失巢凋亡的影响[J]. 江苏大学学报:医学版, 2012, 22(4): 324-327.
CUI Fei-Lun-1, 2 , Qiu-Zhen-1, Hu-Jian-Peng-1, Fan-Yu-2. Studiesof siRNA MTA1 on anoikis of human bladder cancer cell line BIU-87. Journal of Jiangsu University(Medicine Edition), 2012, 22(4): 324-327.
2012, Vol. 22 
