Abstract：Objective: To observe nerve growth factor (NGF) expression in small cell lung cancer cell line NCI-H466, and explore the possible machanism of its brain metastasis. Methods: Subcellular expression of NGF in NCI-H466 was observed by immunofluorescence staining. MTT and transwell assay were used to evaluate the proliferation and invasion of the cell treated by NGF. Results: The subcellular localization of NGF was cell cycle specific. NGF was located in the nuclei at interphase and on spindle at mitotic phase. Administration of NGF promoted the cell proliferation and invasion. Conclusion: the dependence on NGF may account for the tendency of brain metastasis. The subcellular localization of NGF may be explored as a new target for therapy.
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2012, Vol. 22 
