用于炎症治疗的磷脂酰丝氨酸修饰胞外囊泡纳米递药系统的初步构建与评价

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	闫洁
	常恬
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	陈九源
	霍强
	程秀

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Abstract：］Objective： Preliminarily to construct and evaluate phosphatidylserine (PS)-modified macrophage-derived extracellular vesicles (EVs) nanocarriers (PS-EVs@Que) for delivering quercetin (Que) and study the role of PS-EVs@Que in the treatment of inflammation. Methods： EVs were isolated from macrophages by ultracentrifugation, Que was loaded into EVs by ultrasonication (EVs@Que), and finally PSEVs@Que was prepared by incubating PS into EVs@Que solution, the morphology, particle size, drug loading capacity and encapsulation efficiency of formulations were also evaluated; cellular uptake of EVs and PS-modified EVs were determined by immunofluorescence. Macrophages were treated with LPS, LPS+EVs@Que, LPS+PSEVs@Que and control group respectively, and the expression of iNOS, a marker on the surface of macrophages, were detected by Western Blotting; the expression of inflammatory factors TNF-α and IL-6 were detected by Enzyme-Linked Immunosorbent Assay (ELISA). Results： The prepared PS-EVs@Que presented a circular-like vesicle structure with an average particle size of (118.45±0.97) nm, drug loading capacity of (8.59±0.16)% and encapsulation efficiency of (20.08±0.52)%. The uptake results showed that the modification of PS resulted in better phagocytosis of EVs by macrophages and enhanced the uptake of EVs by macrophages. Macrophage iNOS protein expression reduced in the PS-EVs@Que group compared with other groups (P<0.01), and the expression of inflammatory factors TNF-α and IL-6 were significantly inhibited (P<0.01). Conclusion： In this study, we successfully constructed and evaluated a nanomedicine delivery system, which improved the bioavailability of Que, inhibited the polarization of pro-inflammatory phenotype (M1 type) of macrophages, and reduced the pro-inflammatory factors, providing a reference for novel nanomedicines treatment of inflammatory diseases.

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收稿日期: 2023-05-18

基金资助: 安徽省教育厅重大项目（KJ2020ZD52）；安徽省自然科学基金面上项目（1908085MH292）；高校优秀青年人才支持计划项目（gxyq2019038）；蚌埠医学院省重点科研平台开放课题（2023SYKFZ02）；蚌埠医学院科研创新计划项目（Byycx22055）

通讯作者: 霍强（通讯作者），教授，硕士生导师，E-mail： huoqiang@bbmc.edu.cn，程秀（通讯作者），副教授，硕士生导师，Email： 0900023@bbmc.edu.cn

作者简介: 闫洁（1999—），女，硕士研究生

引用本文:

闫洁，常恬，章竟，葛广荣，陈九源，霍强，程秀. 用于炎症治疗的磷脂酰丝氨酸修饰胞外囊泡纳米递药系统的初步构建与评价[J]. 江苏大学学报:医学版, 2024, 24(01): 79-83. YAN Jie, CHANG Tian, ZHANG Jing, GE Guangrong, CHEN Jiuyuan, HUO Qiang, CHENG Xiu. Preliminary construction and evaluation of a phosphatidylserine-modified extracellular vesicles nano-drug delivery system for inflammation therapy

. Journal of Jiangsu University(Medicine Edition), 2024, 24(01): 79-83.

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http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2024/V24/I01/79

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