Abstract:Objective: To explore the effects of scorpion peptide preliminary extract (peptide extract from scorpion tail, PEST) and the centipede preliminary peptide extract (peptide extract from centipede head, PECH) on Hepatitis B virus synthesis and potential mechanism in liver cancer cells. Methods: Ultrafiltration was used to extract PEST and PECH. HepAD38 and HepG2 cells were selected and divided into control group, cells were cultured with high sugar medium containing with 10% fetal bovine serum; PEST group, cells were treated with different concentrations (0.125, 0.250, 0.500 and 1.000 mg/mL) of PEST; PECH group, cells were treated with different concentrations (0.125, 0.250, 0.500,1.000 mg/mL) of PECH; positive control group, cells were treated with lamivudine or entecavir or tetracycline; MTT assay was used to detect cell viability. The qRTPCR was used to detect Hepatitis B virus DNA content in the supernatant of HepAD38 cells. In addition, HepAD38 cells were divided into PEST group (cells were treated with 1 mg/mL PEST), PECH group (cells were treated with 1 mg/mL PECH), control group, positive control group and negative group; HBsAg and HBeAg content were detected by ELISA; HBV X,S,preC,P mRNA relative expression level were detected by qRTPCR; Hepatitis B virus core protein synthesis was detected by Western blotting. Results: The cell viability of HepG2 and HepAD38 cells treated with PEST or PECH were both above 70%;compared with the control group, PEST(0.250, 0.500 and 1.000 mg/mL) or PECH (0.125, 0.500 and 1.000 mg/mL) group showed significantly decreased Hepatitis B virus DNA copy number (all P<0.05); compared with the control group, PEST or PECH group showed significantly lower content of HBsAg and HBeAg and expression level of Hepatitis B virus P mRNA (all P<0.05), PEST group had almost no effect on core protein expression, while PECH group decreased Hepatitis B virus core protein expression. Conclusion: PEST and PECH exerted antiHepatitis B virus effect on HepAD38 cell, which may be related to its inhibition on P mRNA synthesis of Hepatitis B virus.
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