Abstract：Objective: To construct the recombinant luciferase reporter plasmid carrying high mobility group box2(HMGB2) promoter, to identify the activity of the recombinant luciferase reporter plasmid, and to forecast the target transcription factors which can bind to the promoter. Methods: The fragment sequence of HMGB2 promoter, searched from NCBI Genbank database and amplified through PCR was cloned into luciferase reporter plasmid, pGL3-Basic, by digestion with restriction endonucleases and insertion with ligase. The activity of HMGB2 promoter plasmid was detected by luciferase reporter assay. The transcription factors which could bind to the promoter was forecasted by an online software PROMO. Results: The recombinant luciferase reporter plasmid carrying pGL3-HMGB2-promoter was constructed successfully by examination with enzyme digestion and sequencing. The result of luciferase reporter assay indicated that the recombinant luciferase reporter plasmid had transcript activity. With the help of the online software PROMO, 72 transcription factors were forecasted to bind to the promoter. Conclusion: The pGL3-HMGB2-promoter plasmid has the promoter activity and 72 transcription factors may bind to the promoter and promote the expression of HMGB2. ［Key words］high mobility group box2; promoter; luciferase reporter assay; transcription factors
收稿日期: 2018-02-22
通讯作者:
卢春(通讯作者)，教授，博士生导师，E-mail: clu@njmu.edu.cn
引用本文:
王飞, 冯奇, 秦杰, 李婉, 卢春. HMGB2启动子报告基因载体的构建、活性验证及与其结合的转录因子的预测[J]. 江苏大学学报:医学版, 2008, 28(03): 195-199,204.
WANG Fei, Feng-Qi, Qin-Jie, Li-Wan, Lu-Chun. Construction and identification of the activity of HMGB2 promoter reporter vector and forecast of its binding transcription factors. Journal of Jiangsu University(Medicine Edition), 2008, 28(03): 195-199,204.
2008, Vol. 28 
