Abstract：Objective: To fabricate multifunctional oral probiotics microcapsules with CT realtime tracking drug delivery. Methods: Cs/al@BaSO4 microspheres were fabricated using alginate and NaSO4 as injection solution and BaCl2 solution as receiving solution by onestep electrostatic spraying method. Then, these microspheres were coated with the chitosan and immersed into the sodium citrate solution. To investigate the protection against bacteria death and release capabilities of Cs/al@BaSO4 microcapsules loaded Lactic acid bacteria, they were immersed into simulating gastric acid and intestine fluid. To validate the therapeutic effect of dextran sulfate sodium (DSS)induced model of mouse enteritis by Cs/al@BaSO4 microcapsules loaded Lactic acid bacteria through oral administration, measurement of body weight, colon length and histological changes were conducted. The excellent visibility of Cs/al@BaSO4 microcapsules by CT was used to observe the Lactic acid bacteria migration in the digestive. Results: Uniform Cs/al@BaSO4 microcapsules were successful fabricated and their average size was 200 μm.The shell of Cs/al@BaSO4 microcapsules possessed layerbylayer structure, and internal bismuth precipitation with regular crystal structure. Cs/al@BaSO4 microcapsules have superior protection of Lactic acid bacteria in pH 2.5 simulated gastric acid and intestinal targeting release. The treatment of Lactic acid bacteria encapsulated by intestinaltargeted Cs/al@BaSO4 microcapsules could relieve DSSinduced colitis,including increasing the body weight of mice, the length of the colon, intestinal inflammatory cells gradually returned to normal status. Conclusion: The multifunctional Cs/al@BaSO4 microcapsules were successfully fabricated and promoted viability of probiotics after oral administration.Furthermore, they provide an effective visualization tool for probiotics research in vivo.
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2017, Vol. 27 
