Influence of ZIC1 gene combined with curcumin on biological behavior of human breast cancer MDAMB231 cell
SHI Chun-tao1, YU Ming1, WANG Hua-bing1, HAN Wei1, CAO Fang2, DING Hou-zhong2
（1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of General Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan Jiangsu 215300, China)
Abstract：
[Abstract]Objective: To investigate the influence of ZIC1 gene combined with curcumin on biological behavior of human breast cancer cell MDAMB231. Methods: Lentivirus vector pLV-Zic1-PGK-Puro was constructed and transfected stably into MDA-MB231 cell，Western blotting were applied to detect the expression of ZIC1 protein in stably transfected MDA-MB-231 cell.For the following experiments,MDAMB231 cell with no ZIC1 empty vector that we adding curcumin was defined as control group,and the groups of empty vector with adding curcumin,ZIC1 vector with no curcumin,ZIC1 vector with adding curcumin were used as experiment group.MTT assay was used to detect the effect of curcumin(various concentration) on cell proliferation,the standard concentration of curcumin was used in cell adhesion experiment and cell migration experiment when inhibition ratio was 50%，the two experiments were applied to detect differences of cell adhesion rate and migration ability. Cell apoptosis was then tested by FCM assay. Results: Expression of ZIC1 protein were positive in experiment group.The curcumin concentration was 5 mg/L when inhibition ratio was 50%.Compared with control group,ZIC1,curcumin,combination with ZIC1 and curcumin groups showed markedly cell proliferation repression and apoptosis induction(both P＜0.05）.And then，compared with the groups of empty vector with adding curcumin,ZIC1 vector with no curcumin,the effect of experiment group（adding curcumin） for cell proliferation repression and apoptosis induction was more obvious(P＜0.05). Conclusion: The combination with transfection of ZIC1 gene and curcumin worked most effectively in breast caner MDA-MB-231 cell proliferation repression and apoptosis induction,so ZIC1 gene and curcumin maybe has synergistic effect on tumorsuppressor.
史春桃1， 于民1， 汪华兵1， 韩玮1， 曹方2， 丁厚中2. ZIC1基因转染联合姜黄素对人乳腺癌细胞生物学行为的影响[J]. 江苏大学学报:医学版, 2016, 26(02): 113-118.
SHI Chun-tao1, YU Ming1, WANG Hua-bing1, HAN Wei1, CAO Fang2, DING Hou-zhong2. Influence of ZIC1 gene combined with curcumin on biological behavior of human breast cancer MDAMB231 cell. Journal of Jiangsu University(Medicine Edition), 2016, 26(02): 113-118.
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2016, Vol. 26 
