High performance liquid chromatography for simultaneous determination of ergosterol and 22,23dihydroergosterol in Flammulina velutipes
YI Cheng-xue1,2, TONG Shan-shan2, XU Xi-ming2, YU Jiang-nan2
（1. Department of Clinical Laboratory, Zhenjiang Center for Disease Control and Prevention, Zhenjiang Jiangsu 212004; 2. School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013, China)
Abstract：Objective: To develop an efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of ergosterol and 22,23dihydroergosterol in Flammulina velutipes. Methods: Chromatographic separation was achieved on an Inertsil ODS-SP (250 mm×4.6 mm, 5 μm) analytical column using a mobile phase of 100% methanol at 1.0 mL·min-1. The sterol content was detected at 282 nm. The temperature of column was 25 ℃. Results: The established linearity range for ergosterol and 22,23-dihydroergosterol was 0.025-1.5 μg (r=0.999 9). The average recoveries (n=6) were 104.6% with RSD 0.89%,103.5% with RSD 3.34%,respectively. Conclusion: The established HPLC method was simple,rapid and reliable for the separation and simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes.
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2014, Vol. 24 
