肺腺癌转移相关转录子-1和核因子-κB在食管鳞癌组织中的表达及其相关性

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摘要目的: 研究肺腺癌转移相关转录子-1（metastasisassociated lung adenocarcinoma transcript 1，MALAT-1）和核因子-κB（NF-κB）在食管鳞状细胞癌（鳞癌）组织中的表达及其相关性。方法: 采用qRT-PCR技术检测60例食管鳞癌组织和癌旁组织中MALAT1和NFκB的mRNA水平的表达，免疫组化SP方法检测NF-κB的蛋白表达水平。统计分析MALAT1及NFκB与患者临床病理特征之间的关系以及两者的相关性。结果： MALAT-1和NF-κB mRNA在60例食管鳞癌组织中的表达均高于癌旁组织（Z=－3.600，P<0.001；Z=－2.856，P<0.05），且NFκB蛋白在食管鳞癌组织中的阳性表达率显著高于癌旁组织（χ2=36.39, P<0.001）。MALAT-1、NF-κB的表达均与食管鳞癌的分化程度、淋巴结转移以及TNM分期密切相关（P<0.05）。MALAT1与NFκB之间存在正相关（r=0.795，P<0.001）。结论：长链非编码RNA MALAT1可能在食管鳞癌的发生发展中发挥重要作用。

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关键词 ：肺腺癌转移相关转录子-1, 核因子-&kappa, B, 食管鳞状细胞癌, 临床病理特征

Abstract：Objective: To evaluate the expression and correlations of metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1）， and NF-κB in the esophageal squamous cell carcinoma(ESCC) tissue. Methods: The expressions of MALAT-1 and NF-κB mRNA were detected by qRT-PCR in 60 ESCC tissues and adjacent non-tumorous tissues. The expression of NF-κB protein was detected by immunohistochemical staining (SP method), and the relationship between MALAT-1 and clinico-pathological feature of ESCC patients was analyzed, and the correlation was explored. Results: The expression of MALAT-1 and NF-κB both were higher in ESCC tissues than that in adjacent nontumorous tissues (Z=－3.600，P<0.001；Z=－2.856，P<0.05). The positive rate of NF-κB protein in ESCC tissues was significantly higher than that in adjacent nontumor tissues (χ2=36.39, P<0.001). Meanwhile, the higher expression of MALAT-1 and NF-κB were correlated with the differentiation degree, lymphnode metastasis and clinical stage of ESCC (P<0.05).And the expression of MALAT-1 and NF-κB was positive correlated (r=0.795，P<0.001). Conclusion: MALAT-1 might play a significant role in the occurrence and development of ESCC.

收稿日期: 2013-11-27

基金资助:

镇江市社会发展支撑计划项目（SH2011019，SH2012023）

通讯作者: 陈德玉（通讯作者），教授，主任医师，E-mail：cdeyu@hotmail.com

作者简介: 尹越（1988—），女，硕士研究生

引用本文:

尹越1，张曌玥1，牟笑1，吴诗1，毛朝明2，陈德玉2. 肺腺癌转移相关转录子-1和核因子-κB在食管鳞癌组织中的表达及其相关性[J]. 江苏大学学报:医学版, 2014, 24(02): 129-133. YIN Yue1, ZHANG Zhao-yue1, MOU Xiao1, WU Shi1, MAO Chao-ming2, CHEN De-yu2. Expressions and correlations of lncRNA MALAT-1 and NF-κB in patients with esophageal squamous cell carcinoma. Journal of Jiangsu University(Medicine Edition), 2014, 24(02): 129-133.

链接本文:

http://zzs.ujs.edu.cn/xbyxb/CN/ 或 http://zzs.ujs.edu.cn/xbyxb/CN/Y2014/V24/I02/129

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