Abstract:Objective: To analyze the rpoS gene in Serratia plymuthica G3 using bioinformatics approaches to unravel its nucleotide and amino acid sequences,physics, chemistry and structure features with hope in providing help in further studies of the biological function of RpoS in G3.Methods: A series of bioinformatics software were used to predict information on sequence, physical, biochemistry and structure features of RpoS in G3. Results: RpoS was a hydrophilic protein without trans-membrane domain or N-signal peptide. Further analysis revealed that RpoS contained two N-glycosylation sites and 22 phosphrylation sites with a primary secondary structure of α-helix and random coil. Conclusion: The sigma subunit RpoS in strain G3 characterized by a high level of phosphrylation and the secondary structure composed of α-helix and random coil was consistent in its biological function as a signal integrator and network master regulator to be involved in the general stress response in bacteria. [Key words]Serratia plymuthica; RpoS; general stress response; master regulator; bioinformatics