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| Noval magnetic nanoparticles for trypsin immobilization |
| School of Food and Biological Engineering, Jiangsu University, Zhenjiang，Jiangsu 212013，China |
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Abstract The trypsin was successfully immobilized on the surface of magnetic carboxymethyl chitosan (Fe3O4 (PEG+CM-CTS)) nanoparticles by electrostatic interaction. The effects of the medium pH, the concentration of trypsin and the immobilization time on trypsin loading and relative activity onto the magnetic nanoparticles were discussed. The results show that the adsorption equilibrium of trypsin onto the Fe3O4 (PEG+CM-CTS) magnetic nanoparticles well fits Langmuir model, and the maximum trypsin loading is 117.6 mg?g-1 with relative trypsin activity of 87.9 %. The stability experiments indicate that the immobilized trypsin has good operational stability for the continuous hydrolysis of BSA solution with improved storage stability.
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Received: 26 February 2014
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2015, Vol. 36 
