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GC fingerprint and cluster analysis of supercritical CO2 extracts from propolis |
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China) |
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Abstract Supercritical CO2 extracts of propolis (SEP) is the third generation of propolis products. The aim of this study was to establish the fingerprint of SEP with the method of gas chromatography (GC) to control the quality of propolis products. The 16 propolis samples were collected from 9 provinces of China as follows: Hainan, Hebei, Henan, Heilongjiang, Hubei, Hunan, Sichuan, Yunnan and Shandong. The chromatograms obtained from the 16 SEPs include nine common peaks. Their atlas information makes up of the SEP fingerprints. Compared with the control map, the similarities are greater than 0.8. Methodological study results show that the RSD values in five major peaks of retention time are less than 0.8% in the test precision, reproducibility and stability. The fingerprint of SEP include all of the representative propolis in China. Cluster analysis show that the SEP feature ingredients in Hainan, Hebei and Shandong provinces are similar, those of Hubei and Yunnan provinces are similar, those of Hunan province have their own characteristics, and the cluster analysis features of Henan, Heilongjiang and Sichuan provinces are not distinctive.
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