Abstract：In order to find new method of non-clToinjury cryopreservation, the influence of magnetic field on ice crystal formation proceess in KMnO4 solution was studied. Different kinds of magnetic generators were applied, such as rotary magnetic field, megnetic field of square wave superposed sine wave, and 50 kHz magnetic field. In order to achieve a slow and even cooling process, a temperature measuring and controling system was developed by applying virtual instrument technology. The shape of ice crystal influenced by magnetic field was recorded by cryo-microscopc instrument. It is showed that low frequency rotary magnetic field affects the ice crystal formation. Especially, 50 kHz alternated magnetic field can significantly suppress the ice crystal growth in KMnO4 solution. This result is very important in the research of non-cryoinjury cryopreservation of organism influenced by special magnetic field in enviroment with slow temperature dropping rate which is easily obtained, and the result is suitable for real application.
2008, Vol. 29 
