Abstract: In order to solve the shortcoming of relatively low signal-to-noise ratio of coating configurations (dual-layer coating and single-layer coating) in luminescent photoelastic coating method for experimental strain analysis, a new luminescent photoelastic coating configuration was introduced.Two luminescent photoelastic coatings with different configurations were prepared with identical luminescent dyes of Rhodamine B, photoelastic materials of E-44 Epoxy resin, plasticizers of Dibutyl phthalate and curing agents of Diethylenetriamine. Fluorescence emission spectra of the two luminescent photoelastic coatings were measured by Cary-100 fluorescence spectrophotometer with excitation wavelength of 465 nm. The intensity of the new single-layer luminescent coating at emission wavelength of 600 nm is 3.7 times stronger than that of the original dual-layer luminescent photoelastic coatings. Theoretical analysis and experimental results show that the new luminescent photoelastic coating configuration can remarkably improve signal-to-noise ratio and increase fluorescence emission intensity.