Abstract:The composition and structure characteristics of walnut proteins were investigated by different extracted techniques. The results show that the walnut protein contents are 90% by alkaline extractionisoelectric precipitation technique,and 70% by isoelectric precipitation technique,respectively. The secondary structure of highly ordered α-helix structure is damaged for some extent, while β-turn and disordered structure are increased. Subunits and amino acids of walnut protein by the extracted techniques are not damaged. The two extracted techniques show high protein yield with small damage to protein structure and components.