神经干细胞条件培养液对帕金森病模型神经元损伤的保护作用

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Abstract ［Abstract］Objective： To investigate the protective effect of neural stem cell-conditioned medium (NSC-CM) on 6-hydroxydopamine (6-OHDA)-induced neuronal injury in vitro and in vivo model of Parkinson′s disease(PD) and its potential mechanisms. Methods： The in vivo PD model was established by stereotaxic injection of 6-OHDA into the medial forebrain bundle of SD rats, and NSC-CM was injected into the substantia nigra and striatum for treatment. The loss of dopaminergic neuron (DAN) in the substantia nigra and striatum was detected by immunohistochemical staining. The expression level of mitofusin 1 (Mfn1) in substantia nigra was detected by Western blotting; The PD cell model was established in vitro by treating rat adrenal medulla pheochromocytoma (PC12 cells) with 6-OHDA, and pretreated with NSC-CM. Lactate dehydrogenase (LDH) release and cellular reactive oxygen species (ROS) levels in PD cell model were detected, and the expression level of Mfn1 was detected by Western blotting. The components of NSC-CM were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and analyzed by bioinformatics analysis. Flow cytometry and laser confocal microscopy were used to observe the internalization of neural stem cells (NSCs) mitochondria by PC12 cells. The effects of NSC-CM on the cell viability and mitochondrial fluorescence intensity of PC12 cells were compared before and after removal of mitochondria. Results： After injection of NSC-CM, the loss of DAN was significantly decreased and the expression level of Mfn1 protein was greatly increased in PD rats (P<0.05). After NSC-CM pretreatment, the release of LDH and the level of ROS in the PD cell model were significantly decreased (P<0.05), and the expression level of Mfn1 protein was remarkably increased (P<0.05). LC-MS/MS detected the presence of mitochondrial components in NSC-CM, and observed the internalization of mitochondria in NSC-CM by PC12 cells. Removal of mitochondria from NSC-CM significantly decreased the survival rate of PC12 cells (P<0.05) and the fluorescence intensity of mitochondria (P<0.05). Conclusion： NSC-CM could improve neuronal death, increased oxidative stress level and decreased mitochondrial fusion level caused by 6-OHDA, and this protective effect may be related to PC12 cells internalizing NSCs mitochondria.

Received: 21 March 2022

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	NI Wei1
	ZHOU Jiqin1
	LING Yating1
	LYU Xiaorui1
	NIU Dongdong1
	ZENG Yu1
	QIU Yun1
	SI Yu1
	WANG Ziyu2
	HU Jiabo1

Cite this article:

NI Wei1,ZHOU Jiqin1,LING Yating1等. Protective effect of neural stem cell-conditioned medium on neuronal injury in Parkinson′s disease model[J]. Journal of Jiangsu University(Medicine Edition), 2023, 33(04): 284-291.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2023/V33/I04/284

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