神经干细胞分泌组通过调节小胶质细胞表型抑制体内外神经炎症

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Abstract ［Abstract］Objective： To explore the role of neural stem cell secretome (NSC-S) on neuroinflammation in vitro and in vivo and its potential mechanisms. Methods： Mouse models of neuroinflammation were developed by lipopolysaccharide (LPS), the effects of NSC-S on the behavior of mice were evaluated by open-field test, and the effects of NSC-S on the microglia phenotype and neuronal injury of cerebral cortex were assessed by immunofluorescence staining and Nissl staining. BV2 microglial inflammatory model was developed by LPS stimulation in vitro. The effect of NSC-S on the phenotype of BV2 cells was detected by flow cytometry. The antiinflammatory mechanism of NSC-S was studied by using peroxisome proliferator activated receptor-γ (PPAR-γ) inhibitor GW9662. Results： The total distance and average movement speed of the mice treated with NSC-S were significantly increased in the open field test (P<0.05), NSC-S regulated the phenotypic polarization of microglia and inhibited neuronal injury in mouse cortex. In vitro, NSC-S inhibited the expression of M1 surface marker CD86 (P<0.05) and promoted the expression of M2 surface marker CD206 (P<0.01) in BV2 cells, however, GW9662 blocked the regulation effect of NSC-S on BV2 cells phenotypes (P<0.05). Conclusion： NSC-S plays a protective role in LPS-induced neuroinflammation in vivo and in vitro by regulating microglia phenotypes.

Received: 26 March 2022

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	Articles by authors
	ZHOU Jiqin1
	NI Wei1
	LING Yating1
	LYU Xiaorui1
	NIU Dongdong1
	ZENG Yu1
	QIU Yun1
	SI Yu1
	WANG Ziyu2
	HU Jiabo1

Cite this article:

ZHOU Jiqin1,NI Wei1,LING Yating1等. Neural stem cell secretome inhibited neuroinflammation in vivo and in vitro by regulating microglial phenotypes[J]. Journal of Jiangsu University(Medicine Edition), 2023, 33(04): 277-283.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2023/V33/I04/277

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