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Abstract Objective： To study the effects of bone marrow mesenchymal stem cells （BMMSCs） infusion on plasma C3 and IgG concentrations， and CXCR4 expression of B cells and plasma cells in MRL/lpr lupus mice， and to explore the mechanism of BMMSCs in regulating disease activity of systemic lupus erythematosus. Methods： BMMSCs were isolated from C57BL/6 mice and cultured， and then injected into MRL/lpr lupus mice by tail transfusion. All mice were divided into C57BL/6 group （C57BL/6 mice were not injected ）， MRL/lpr group （MRL/lpr mice were not injected with BMMSCs）， BMMSCs infusion group （MRL/lpr mice were injected with BMMSCs at 12 weeks）， and PBS control group （MRL/ lpr mice were injected with PBS at 12 weeks）.At the end of the 20th week， peripheral blood， bone marrow and spleen of mice were taken for flow cytometry analysis to detect CXCR4 expression in B and plasma cells， and then the plasma of the 4 groups were taken for ELISA to detect IgG and C3 level. The kidney of mice was stained with hematoxylineosin. Results： ① Compared with the C57BL/6 group， the plasma C3 level were decreased and IgG level were significantly increased in the MRL/lpr group（all P<0.01）. Compared with C57BL/6 group， the expression of CXCR4 on B cell surface in MRL/lpr group was significantly decreased in peripheral blood， and was significantly increased in bone marrow and spleen（all P<0.01）. The proportion of CXCR4 on plasma cell surface in bone marrow（P<0.05） and spleen（P<0.01） of MRL/lpr group was significantly higher than that of C57BL/6 mice.② Compared with PBS or MRL/lpr group， the level of C3 in BMMSCs group was significantly increased， while the level of IgG was significantly decreased（all P<0.01）. Compared with the PBS group， the expression of CXCR4 on B cell surface of BMMSCs group was significantly increased in peripheral blood（P<0.01）， and significantly decreased in bone marrow（P<0.01） and spleen（P<0.05）. Compared with the MRL/lpr group， the expression of CXCR4 on B cell surface of bone marrow and spleen was significantly decreased （all P<0.01）.③ Compared with PBS group or MRL/lpr group， the expression of CXCR4 on plasma cell surface in bone marrow and spleen of BMMSCs group was significantly decreased（all P<0.05）.④ Renal HE staining suggested attenuation of renal inflammation in MRL/lpr mice after infusion of BMMSCs. Conclusion： BMMSCs infusion can regulate the expression of CXCR4 on the surface of B and plasma cells and improve the disease activity index of MRL/lpr mice.

Received: 28 January 2021

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	Articles by authors
	MA Cui
	TANG Yu
	CHENG Mengwei
	SHI Wei
	ZHENG Wenjuan
	QIU Yingying
	RUI Jinbing
	WANG Yanru

Cite this article:

MA Cui,TANG Yu,CHENG Mengwei等.

Effects of bone marrow mesenchymal stem cells infusion on CXCR4 expression in B cells of MRL/lpr lupus mice

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 406-411.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I05/406

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