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Expression of Quaking isoform 7 in breast carcinoma and its relationship with prognosis of breast carcinoma patients |
| XU Zhen-lei1, WANG Qing-wei2 |
| (1. Department of Burn and Plastic Surgery, 2. Department of Laboratory Medicine, The Affiliated Huai′an No.1 People′s Hospital of Nanjing Medical University, Huai′an Jiangsu 223001, China)
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Abstract Objective: To investigate the expression of Quaking isoform 7(QKI-7) protein in breast carcinoma tissue and its clinical significance. Methods: The positive expression rate and intracellular localization of QKI-7 were detected in breast carcinoma and paracancerous tissues by immunohistochemical staining; correlation of QKI-7 expression with tumor tissues and clinical pathological parameters of patients, followup data, P53 and Ki67 expression were analyzed. Results: The positive rate of QKI-7 in breast carcinoma tissues was significantly lower than that in paracancerous tissues(χ2=73.378, P<0.05). The expression of QKI-7 in breast carcinoma tissue was related to tumor size, lymph node metastasis and TNM stage(all P<0.05). Cumulative survival rate in QKI-7 negative patients was significantly lower than that in QKI-7 positive patients(P<0.05); the expression of QKI7 in breast carcinoma tissue was positively correlated with P53 expression, while negatively correlated with Ki-67 expression(all P<0.05). Conclusion: The low expression of QKI7 may indicate poor prognosis of breast carcinoma patients.
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Received: 05 July 2018
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| ［1］Darbelli L, Richard S. Emerging functions of the Quaking RNAbinding proteins and link to human diseases\[J\]. Wiley Interdiscip Rev RNA, 2016, 7(3):399-412.［2］Li S, Lin C, Zhang J, et al. Quaking promotes the odontoblastic differentiation of human dental pulp stem cells\[J\]. J Cell Physiol, 2018, 233(9):7292-7304.［3］Darbelli L, Vogel G, Almazan G, et al. Quaking regulates neurofascin 155 expression for myelin and axoglial junction maintenance\[J\]. J Neurosci, 2016, 36(14): 4106-4120.［4］Hall MP, Nagel RJ, Fagg WS, et al. Quaking and PTB control overlapping splicing regulatory networks during muscle cell differentiation\[J\]. RNA, 2013, 19(5):627-638.［5］Darbelli L, Choquet K, Richard S, et al. Transcriptome profiling of mouse brains with QKIdeficient oligodendrocytes reveals major alternative splicing defects including selfsplicing\[J\]. Sci Rep, 2017, 7(1):7554-7566.［6］Iwata N, Ishikawa T, Okazaki S, et al. Clinical significance of methylation and reduced expression of the quaking gene in colorectal cancer\[J\]. Anticancer Res, 2017, 37(2):489-498.［7］De Miguel FJ, Pajares MJ, MartinezTerroba E, et al. A largescale analysis of alternative splicing reveals a key role of QKI in lung cancer\[J\]. Mol Oncol, 2016, 10(9):1437-1449.［8］Lu W, Feng F, Xu J, et al. QKI impairs selfrenewal and tumorigenicity of oral cancer cells via repression of SOX2\[J\]. Cancer Biol Ther, 2014, 15(9):1174-1184.［9］Bian Y, Wang L, Lu H, et al. Downregulation of tumor suppressor QKI in gastric cancer and its implication in cancer prognosis\[J\]. Biochem Biophys Res Commun, 2012, 422(1):187-193.［10］Zhang RL, Yang JP, Peng LX, et al. RNAbinding protein QKI5 inhibits the proliferation of clear cell renal cell carcinoma via posttranscriptional stabilization of RASA1 mRNA\[J\]. Cell Cycle, 2016, 15(22):3094-3104.［11］Zhao Y, Zhang G, Wei M, et al. The tumor suppressing effects of QKI5 in prostate cancer: a novel diagnostic and prognostic protein\[J\]. Cancer Biol Ther, 2014, 15(1):108-118.［12］Yoo YG, Kong G, Lee MO. Metastasisassociated protein 1 enhances stability of hypoxiainducible factor1α protein by recruiting histone deacetylase 1\[J\]. EMBO, 2006, 25(6):1231-1241.［13］Zong FY, Fu X, Wei WJ, et al. The RNAbinding protein QKI suppresses cancerassociated aberrant splicing\[J\]. PLoS Genet, 2014, 10(4):e1004289.［14］Zhou L, Zhou W, Zhang H, et al. Progesterone suppresses triplenegative breast cancer growth and metastasis to the brain via membrane progesterone receptor α\[J\]. Int J Mol Med, 2017, 40(3):755-761.［15］Li J, Zhang X, Zheng L, et al. Association of nuclear FOXP3 expression with low Ki67 index and better prognosis in patients with breast invasive ductal carcinoma\[J\]. Neoplasma, 2017, 64(5):754-761.［16］Duffy MJ, Synnott NC, Mcgowan PM, et al. P53 as a target for the treatment of cancer\[J\]. Cancer Treat Rev, 2014, 40(10):1153-1160. |
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2019, Vol. 29 
