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| Optimization of gestational diabetes mellitus induced
by highfat diet combined with streptozotocin |
| TANG Meng-yu, XU Wen-ting, WANG Cai-hong, WANG Li-hong |
| (Reproductive Department, Zhangjiagang Hospital of Traditional Chinese Medicine, Suzhou Jiangsu 215600, China)
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Abstract Objective: To explore the reliable method of establishing the rat model of gestational diabetes with high fat feed combined with streptozotocin(STZ), and to establish the best dose of stable STZ rat model of gestational diabetes mellitus. Methods: The 6week old SPF rats were fed a highfat diet for 8 weeks after one week of adaptive feeding.According to the ratio of two females to one male, the next day vaginal smear was examined, if the sperm was found D1 day pregnant was recorded. The pregnant rats were randomly divided into control group and STZ groups (15,25,35,45 mg/kg).They were given intraperitoneal injection of the same volume of citric acid buffer (0.1 mol/L, pH=4.2) or STZ 15, 25, 35, 45 mg/kg respectively.Body weight, fasting blood glucose and the rate of moled formation, mortality, offspring were measured or observed. In addition the pancreas, liver and adipose tissue in pregnant rats were assessed by HE staining. Results: The moleding rate of 25 mg/kg group was 62.5%, which was the highest,and the death rate of 12.5% is the lowest.After injection of STZ, blood glucose level of 25,35 and 45 mg/kg groups increased significantly, and the blood level of 25 mg/kg group was more stable. Conclusion: SPF grade 6week old Wistar rats were fed highfat diet for 8 weeks and STZ at the dosage of 25 mg/kg was injected on the first day of gestation, which was a reliable way to establish a stable model of gestational diabetes mellitus in rats.
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Received: 31 May 2018
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2018, Vol. 28 
