B7共刺激分子在恶性血液病细胞株中的表达特征

Abstract
Figure/Table
References
Related Citation (3)

Download: PDF (1824 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the expression and distribution characteristic of B7 costimulatory molecules in human hematologic malignancy cell lines. Methods: The expression and molecules subcellular distribution of B7.1, B7.2, HLA classⅠ, HLA classⅡ, and B7H1, CD279, B7H3 and B7H4 in 13 human hematologic malignancy cell lines were determined by flow cytometry, with the peripheral blood mononuclear cells(PBMCs) of 12 volunteers as the control. Results: Multiple human hematologic malignancy cell lines expressed low or undetectable level of B7.1 on the cell membrane, detectable or high level of B7.2, and high or detectable level of HLA classⅠand Ⅱ molecules. The membrane proteins of B7H1, CD279, B7H3 and B7H4 had low or no expression in cell lines, while the expression abundance of B7H3 was higher in U937, Maver and Z138 cell lines. The cytoplasmic protein of B7H1, B7H3 and B7H4 almost had no expression in cell lines, while the relative nuclear and cytoplasmic protein were abnormal expression or high expression in these cell lines, except for CZ1. Conclusion: There were significant differences among B7 molecules expression in both hematologic malignancy cell lines with different types, and the cell lines derived from the same type of tumor, which might be involved in the immune escape, the occurrence and development of hematological malignancies.

Received: 12 July 2018

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I05/386

［1］Greaves P,Gribben JG.The role of B7 family molecules in hematologic malignancy\[J\]. Blood, 2013,121(5):734-744.

［2］Seliger B, Quandt D. The expression, function, and clinical relevance of B7 family members in cancer\[J\]. Cancer Immunol Immunother, 2012,61(8):1327-1341.

［3］Reeves E, James E. Antigen processing and immune regulation in the response to tumours\[J\]. Immunology, 2017,150(1):16-24.

［4］Garrido F, RuizCabello F, Aptsiauri N. Rejection versus escape: the tumor MHC dilemma\[J\]. Cancer Immunol Immunother, 2017,66(2):259-271.

［5］Satelli A, Batth IS, Brownlee Z, et al. Potential role of nuclear PDL1 expression in cellsurface vimentin positive circulating tumor cells as a prognostic marker in cancer patients\[J\]. Sci Rep, 2016,6:28910.

［6］Ingebrigtsen VA,Boye K,Nesland JM,et al.B7H3 expression in colorectal cancer:associations with clinicopathological parameters and patient outcome\[J\].BMC Cancer, 2014,14:602.

［7］Chen C, Qu QX,Xie F, et al. Analysis of B7H4 expression in metastatic pleural adenocarcinoma and therapeutic potential of its antagonists\[J\].BMC Cancer,2017, 17:652.

［8］贾丽萍, 克晓燕, 王晶. B7共刺激分子在八种人类恶性血液病细胞系中的表达及意义\[J\]. 中华血液学杂志, 2002，23(7):345-348.

［9］Tada K, Maeshima AM, Hiraoka N, et al. Prognostic significance of HLA class Ⅰ and Ⅱ expression in patients with diffuse large B cell lymphoma treated with standard chemoimmunotherapy\[J\]. Cancer Immunol Immunother, 2016,65(10):1213-1222.

［10］Nijland M, Veenstra RN, Visser L, et al. HLA dependent immune escape mechanisms in Bcell lymphomas: Implications for immune checkpoint inhibitor therapy?\[J\]. Oncoimmunology, 2017,6(4):e1295202.

［11］Andorsky DJ, Yamada RE, Said J, et al. Programmed death ligand 1 is expressed by nonhodgkin lymphomas and inhibits the activity of tumorassociated T cells\[J\]. Clin Cancer Res, 2011, 17(13): 4232-4244.

［12］Guery T, Roumier C, Berthon C, et al. B7H3 protein expression in acute myeloid leukemia\[J\]. Cancer Med, 2015,4(12):1879-1883.

［13］Che F, Heng X, Zhang H, et al. Novel B7H4mediated crosstalk between human nonHodgkin lymphoma cells and tumorassociated macrophages leads to immune evasion via secretion of IL6 and IL10\[J\]. Cancer Immunol Immunother, 2017, 66(6):717-729.

［14］张巍, 王晶, 王艳芳, 等. 共刺激分子B7H1、B7H3和B7H4在血液系统恶性肿瘤细胞株中的表达水平和分布特征\[J\]. 中国实验血液学杂志, 2016, 24(5):1539-1546.

［15］Ingebrigtsen VA, Boye K, Tekle C, et al. B7H3 expression in colorectal cancer: nuclear localization strongly predicts poor outcome in colon cancer\[J\]. Int J Cancer, 2012,131(11):2528-2536.