分化抑制因子1基因在急性髓系白血病患者骨髓单个核细胞中的表达

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Abstract Objective: To explore the expression of inhibitor of differentiation1(ID1) gene in bone marrow mononuclear cells and its clinical significance in patient with acute myeloid leukemia(AML). Methods: Realtime quantitative PCR was applied to detect ID1 transcript level in bone marrow mononuclear cells from 153 de novo AML patients. Results: ID1 transcript level in AML patients ranged from 0.000 to 3.536(median 0.030). According to the median level of ID1 expression, AML patients were divided into two groups, i.e. high ID1 expression and low ID1 expression. No significant differences were found in sex, peripheral platelet, and common gene mutations between two groups(P>0.05). There were tendency towards the differences between two groups in peripheral white blood cell, peripheral hemoglobin, FAB subtypes, WHO classifications, karyotypes, and karyotypic risks(P<0.10). However, significant differences were observed in age and percentage of bone marrow blasts between two groups(P<0.05), and high ID1 expression was associated with older age(P=0.033) and higher percentage of bone marrow blasts (P=0.035). Patients with high ID1 expression presented a significantly lower complete remission(CR) rate than those with low ID1 expression(P<0.01), and AML patients who can achieve CR in 12 course of induction chemotherapy showed a significantly lower ID1 expression in newly diagnosis time than those who cannot achieve CR in 12 course of induction chemotherapy(P=0.001). Survival analysis showed that patients with high ID1 expression had a shorter overall survival(OS) than those with low ID1 expression in whole AML, nonM3 AML, and cytogenetically normal AML(P=0.002, 0.008, and 0.050, respectively). Conclusion: The level of ID1 expression may act as a potential biomarker associated with chemotherapy response and prognosis in AML.

Received: 19 October 2017

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2018/V28/I01/65

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