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| IL-36γ enhances human CD8+T lymphocytes activation and promotes cytokines production |
| LV Quan-sheng1， MA Jing-shu1， GU Yan-zheng2 |
| 1. School of Biology ＆ Basic Medical Sciences, Soochow University，Suzhou Jiangsu 215123； 2. Institute of Clinical Immunology, First Affiliated Hospital, Soochow University,Suzhou Jiangsu 215006； |
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Abstract Objective: To investigate whether recombinant interleukin-36γ(rIL-36γ) can enhance human CD8+T lymphocytes secrete cytokine interferon-γ（IFN-γ）in vitro. Methods: Peripheral blood mononuclear cells(PBMC) was isolated from healthy donors by density gradient centrifugation,and CD8+T lymphocytes were purified by positive magnetic beads selection from PBMCs. CD8+T cells were stimulated with different combination of cytokines(CD3 mAb+CD28 mAb；CD3 mAb+CD28 mAb+IL-12；CD3 mAb+CD28 mAb+IL-36γ；CD3 mAb+CD28 mAb+IL-12+IL-36γ) in vitro. The cells of each group were collected after 24 h and 72 h. CD8+T lymphocytes purity and cytokine IFN-γ production were determined by flow cytometry. Real time PCR(qRT-PCR) was used to detect IL-36 receptor(IL36R), IFNγ and granzyme B mRNA expression. Results: Human CD8+T lymphocytes expressed IL36 receptor；there was no significant difference of cell morphology among the four groups of CD8+T lymphocytes；IFN-γ and granzyme B of CD8+T lymphocytes stimulated by IL-36 γ were significantly increased in mRNA levels(P<0.05)；at the same time, IL-36γ and IL-12 had a costimulation role to activate CD8+T lymphocytes. Conclusion: IL-36γ has a synergistic effect with IL-12 to enhance human CD8+T lymphocytes cytokine secretion in vitro.
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Received: 09 March 2016
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2016, Vol. 26 
