基于生物信息学筛选肠易激综合征免疫浸润下的染色质调节因子

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Abstract Objective： To screen out potential chromatin regulators (CRs) under immune infiltration in irritable bowel syndrome (IBS) by bioinformatics analysis and to evaluate their links with immune cells and immune functions. Methods： The CRs file and IBS gene expression matrix were integrated, and weighted gene coexpression analysis and differential analysis were performed to take the intersection CRs; based on the CRs obtained from the intersection, proteinprotein interaction (PPI) analysis was performed and the network diagram was constructed. Immune infiltration was extracted and quantified using a gene expression matrix to analyze the correlation and variability between immune cells and between immune functions; and the correlation with CRs in the PPI network diagram was analysed. By constructing a risk model, we analyzed the risk probability of CRs closely related to immune infiltration, and thus screened out the potential CRs. Results： PPI analysis identified a total of 13 interacting CRs in IBS. Among immune cells, the correlation ship between B cells and follicular T helper cells was the strongest and positively correlated (r=0.90). In immune function, the correlation ship between checkpoint and T cell coinhibition was the strongest and positively correlated (r=0.89). Compared with healthy subjects, IBS patients showed a significant increase in co-stimulation of dendritic cells, immature dendritic cells, Th1 cells, and antigen presentation (all P<0.05). The correlation analysis between CRs and immune infiltration showed that SIN3 transcription regulator family member B gene (SIN3B) was positively correlated with natural killer cells, serine/arginine repetitive matrix protein 2 (SRRM2) was positively correlated with Th1 cells and antigen--presenting cells co-stimulation, while DPY30 was negatively correlated with helper T cells and Th1 cells. Risk model results showed that SIN3B and SRRM2 may be independent risk factors for IBS. Conclusion： SIN3B and SRRM2 are potential CRs for immune infiltration in IBS, which may regulate the occurrence and development of IBS by regulating natural killer cells, Th1 cells and antigen presentation co-stimulation.

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Received: 06 October 2022

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	Articles by authors
	PENG Jianlan
	ZHU Yongping
	LIN Shouning
	LIAO Dongyan
	JIANG Junling
	WU Yuexia

Cite this article:

PENG Jianlan,ZHU Yongping,LIN Shouning等. Screening of potential chromatin regulators under immune infiltration in irritable bowel syndrome based on bioinformatics

[J]. Journal of Jiangsu University(Medicine Edition), 2024, 34(02): 166-175.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2024/V34/I02/166

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