邻苯二甲酸二（2乙基己基）酯致人脐静脉内皮细胞氧化损伤及白藜芦醇的修复作用

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Abstract Objective： To investigate the oxidative damage of human umbilical vein endothelial cells HUVEC induced by di-2-ethylhexyl phthalate (di-2-ethylhexyl phthalate, DEHP), and the repairing effect of resveratrol on this damage. Methods： The ranges of damage concentration of DEHP and the repair concentration of resveratrol were screened by CCK8 experiment. The different levels of malondialdehyde (MDA) and superoxide dismutase （SOD） activity in HUVEC were detected by chemical spectrophotometry. The content of reactive oxygen species （ROS） in HUVEC was detected by fluorescent probe of reactive oxygen species. The mitochondrial membrane potential (MMP) of different HUVEC groups was detected by JC-1 kit. CCK8 experiment, Transwell experiment and scratch healing experiment were applied to observe the changes of various cell capacities of HUVEC. The apoptosis of HUVEC were detected by cell flow cytometry experiment and Western blotting. Results： After treated with 80 μmol/L DEHP for 24 hours, the cell proliferation ability was significantly reduced (P<0.01), and 40 μmol/L resveratrol had no obvious toxicity to the cells; The MDA level and SOD activity of the cells in the injury group were significantly higher than those in the control group (all P<0.01), compared with the damage group, the repair group were significantly decreased (all P<0.05); the expression of ROS in the damage group was significantly higher than that in the control group (P<0.01), the repair group was significantly lower than the damage group (P<0.01); the MMP of the damage group was significantly lower than that of the control group (P<0.01), and the repair group was significantly higher than the damage group (P<0.01); Compared with the control group, the proliferation, migration, and tube-forming abilities of the cells were significantly decreased (all P<0.05), and the repair group was significantly increased compared with the damage group (all P<0.05); the apoptosis level of the damage group was significantly higher than that of the control group (P<0.05), the repair group was significantly lower than the damage group (P<0.05). Conclusion： DEHP can inhibit the proliferation, migration, and tube-forming abilities of HUVEC through oxidative stress pathway and induce its apoptosis, while resveratrol can effectively repair the damage.

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