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Oligodendrocytes-like conditional medium can improve oxidative stress damage of SH-SY5Y cells by regulating Sirt1/PGC-α pathway#br# |
NIU Dongdong1, LING Yating1, LYU Xiaorui1, WANG Ziyu2, HU Jiabo1 |
(1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Health Clinical Laboratories, Health BioMed Co. Ltd., Ningbo Zhejiang 315042, China)
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Abstract Objective: To explore the repair effect of oligodendrocyteslike conditioned medium (OCM) derived from human neural stem cells (hNSCs) on human neuron and its possible mechanism. Methods: Human neural stem cells differentiated into oligodendrocytes-like (OLs), which were identified by immunofluorescence and their myelination ability was observed in vitro. The oxidative damage model of SH-SY5Y cells was induced by H2O2. Reactive oxygen species (ROS) and cell apoptosis were determined by flow cytometry. Mitochondrial membrane potential was detected by fluorescence microscopy. SOD, GSH and MDA were detected by chemiluminescence. The protein level of Sirt1/PGC-1α was determined by Western blotting. Results: Human neural stem cellderived oligodendrocytes-like (hNSC-OLs) have a typical oligodendrocyte morphology and express oligodendrocyte markers Olig2 and SOX10, the formation of myelin sheath around axons was observed in vitro. OCM significantly increased the vitality of the damaged cells (P<0.01), decreased ROS level (P<0.01), increased mitochondrial membrane potential, increased GSH, SOD level (P<0.01), decreased MDA content (P<0.01), and decreased apoptosis level (P<0.01). OCM treatment increased the expression levels of Sirt1 and PGC-1α protein (P<0.05), and was specifically inhibited by the inhibitor EX527 (P<0.05). Conclusion: hNSC-OLs have myelination ability in vitro, and its conditioned medium can improve H2O2induced oxidative stress of SH-SY5Y cells and reduce SHSY5Y cell apoptosis through Sirt1/PGC-1α pathway.
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Received: 18 February 2023
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