4种荧光原位杂交探针检测隐匿易位急性早幼粒细胞白血病的比较

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Abstract Objective： To study the detection ability of four commonly used fluorescence in situ hybridization (FISH) probes in cryptic PML-RARα translocation cases. Methods： Combined use of FISH, RT-PCR and conventional karyotyping techniques to detect APL-related fusion genes and chromosomal abnormalities of three insertional APL cases. PML-RARα FISH probes were from four different companies. Results： When use FISH method to detect rare insertional PML-RARα fusions genes, false negatives are prone to occur due to the short fragments. Comparative detection results of four sets of PML-RARα probes showed that all three were false-negative by Abbott probe while one false-negative and two positives by Cytocell probe. Kanglu and Anbiping probes detected fusion signals in all three cases. Conclusion： Kanglu and Anbiping probes had the highest positive detection rate, which were better choice for detecting such rare cryptic PML-RARα fusion genes by FISH technique.

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Received: 16 September 2022

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2023/V33/I01/57

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