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| Effect of total flavonoids of Scutellaria baicalensis Georgi on Camk2d and Ppp3r2 expression in cartilage of knee osteoarthritis rats |
| LIU Dakai 1, ZHENG Xifu 2, LI Anshi 1 |
（1. Department of Joint Surgery, Dalian No.2 People′s Hospital, Dalian Liaoning 116011 ； 2. Department of Orthopaedics Surgery, First Affiliated Hospital of Dalian Medical University, Dalian Liaoning 116011, China）
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Abstract Objective: To investigate the effect of total flavonoids of Scutellaria baicalensis Georgi （TFSB） on calcium/calmodulin dependent protein kinaseⅡdelta（Camk2d） and protein phosphatase 3 regulates subunit 2（Ppp3r2） expression in cartilage of knee osteoarthritis rats. Methods: The knee osteoarthritis model was prepared by injecting papain into the knee joint cavity. The rats in the negative control group and the model group were given normal saline by intragastric administration. Rats in TFSB low-dose group, middle-dose group and high-dose group were given the TFSB by gavage at the doses of 12.5, 25, and 50 mg/kg. The positive control group rats were gavaged with celecoxib （10 mg/kg）, and treated for 14 consecutive days. The pathological knees joint of rats was observed and Makin′s score were performed, and the TNF-α, IL-1β, IL-6, Camk2d and Ppp3r2 of knee cartilage tissue were detected by ELISA. The levels of Camk2d and Ppp3r2 protein in knee cartilage tissue were detected by Western Blotting. Results: In the model group, the knee cartilage fiber connective tissue was proliferated, and the structure was unclear, and the inflammatory cells infiltrated. The low-dose group of TFSB had hyperplasia of articular cartilage fiber connective tissue, irregular cell arrangement and inflammatory cell infiltration, but it was significantly improved compared with the model group. With the increasing of the dose of TFSB, the improvement of the lesion was more obvious. Compared with the negative control group, the Makin′s score, TNF-α, IL-1β, IL6 and Camk2d levels in the knee cartilage tissues of the model group increased, while the Ppp3r2 level decreased（all P<0.05）. After the TFSB or celecoxib treatment, compared with the model group, the Makin′s score, TNF-α, IL-1β, IL-6 and Camk2d levels in each dose group of TFSB and the positive control group decreased, and the level of Ppp3r2 increased, and the effect of each dose group of TFSB was dosedependent（all P<0.05）. The indicators between the high-dose group of TFSB and the positive control group were no significant difference（all P>0.05）. Conclusion: TFSB may exert therapeutic effect in rats with knee osteoarthritis by increasing the expression of Ppp3r2 and reducing the expression of Camk2d in cartilage.
［Key words］total flavonoids of Scutellaria baicalensis Georgi; knee osteoarthritis; calcium/calmodulin dependent protein kinase Ⅱdelta; protein phosphatase 3 regulates subunit 2; rats; cartilage
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Received: 29 December 2021
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