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| concentrations of fetal bovine serum on proliferation and differentiation of primary cadiac fibroblasts in vitro |
| LI Shanshan, DANG Shipeng, ZHANG Zhenye, QIAN Lingling, CHEN Xufei, WU Ying, LI Xiaoyan, TANG Xu, WANG Ruxing |
| （Department of Cardiology, the Affiliated Wuxi People′s Hospital of Nanjing Medical University, Wuxi Jiangsu 214023, China） |
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Abstract Objective: To investigate the effects of different concentrations of fetal bovine serum on proliferation and differentiation of primary cardiac fibroblasts in vitro. Methods: Cardiac fibroblasts were isolated from SD neonatal rats by enzyme digestion and differential adhesion method, then cultured with different concentrations of fetal bovine serum （1%,5%,10% and 20%）. The cell morphology was observed, and the expression of α-SMA protein was detected by immunofluorescence confocal assay. The migration ability of cardiac fibroblasts was detected by scratch assay. The proliferation ability of cardiac fibroblasts was detected by CCK8 assay. Results: With the increase of fetal bovine serum concentration, the cell morphology gradually became round, the cell surface area increased, the proportion of αSMA positive cells increased, the differentiation of cardiac fibroblasts to myofibroblasts increased, and the cell migration ability increased. The proliferation ability of myocardial fibroblasts was the most vigorous when cultured with the highest concentration of fetal bovine serum （20%）. The proliferation ability of myocardial fibroblasts was the lowest when cultured with low concentration of fetal bovine serum （1%）. In 5% and 10% concentration of serum treated cardiac fibroblasts, the proliferation rate of cells was between high and low concentration of fetal bovine serum. Conclusion: Cardiac fibroblasts cultured in high fetal bovine serum concentration can lead to differentiation of myocardial fibroblasts. Compared with 10% and 20% concentration of serum, cardiac fibroblasts cultured in 5% serum concentration have less differentiation. Compared with 1% concentration of serum, cardiac fibroblasts cultured in 5% serum concentration have better proliferation ability. It is better to select 5% serum concentration to culture cardiac fibroblasts.
［Key words］cardiac fibroblasts; in vitro culture; concentrations of fetal bovine serum; α-SMA; differentiation
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| [1] |
. [J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(06): 536-540. |
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