全蝎和蜈蚣的多肽粗提物对肝癌HepAD38细胞中HBV的抑制作用

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Abstract Objective: To explore the effects of scorpion peptide preliminary extract （peptide extract from scorpion tail, PEST） and the centipede preliminary peptide extract （peptide extract from centipede head, PECH） on Hepatitis B virus synthesis and potential mechanism in liver cancer cells. Methods: Ultrafiltration was used to extract PEST and PECH. HepAD38 and HepG2 cells were selected and divided into control group, cells were cultured with high sugar medium containing with 10% fetal bovine serum; PEST group, cells were treated with different concentrations （0.125, 0.250, 0.500 and 1.000 mg/mL） of PEST; PECH group, cells were treated with different concentrations （0.125, 0.250, 0.500，1.000 mg/mL） of PECH; positive control group, cells were treated with lamivudine or entecavir or tetracycline; MTT assay was used to detect cell viability. The qRTPCR was used to detect Hepatitis B virus DNA content in the supernatant of HepAD38 cells. In addition, HepAD38 cells were divided into PEST group （cells were treated with 1 mg/mL PEST）, PECH group （cells were treated with 1 mg/mL PECH）, control group, positive control group and negative group; HBsAg and HBeAg content were detected by ELISA; HBV X，S，preC，P mRNA relative expression level were detected by qRTPCR; Hepatitis B virus core protein synthesis was detected by Western blotting. Results: The cell viability of HepG2 and HepAD38 cells treated with PEST or PECH were both above 70%；compared with the control group, PEST（0.250, 0.500 and 1.000 mg/mL） or PECH （0.125, 0.500 and 1.000 mg/mL） group showed significantly decreased Hepatitis B virus DNA copy number （all P<0.05）; compared with the control group, PEST or PECH group showed significantly lower content of HBsAg and HBeAg and expression level of Hepatitis B virus P mRNA （all P<0.05）, PEST group had almost no effect on core protein expression, while PECH group decreased Hepatitis B virus core protein expression. Conclusion: PEST and PECH exerted antiHepatitis B virus effect on HepAD38 cell, which may be related to its inhibition on P mRNA synthesis of Hepatitis B virus.

Received: 13 May 2020

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	Articles by authors
	MA Qing1
	YANG Yang2
	MA Tao3
	XU Weidong1
	ZHAO Ming1

Cite this article:

MA Qing1,YANG Yang2,MA Tao3等.

Inhibition of Hepatitis B virus in liver cancer cells by preliminary peptide extracts of scorpion and centipede

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 431-437.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I05/431

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