自拟调气汤对顺铂诱导的大鼠肾损伤的影响及其机制

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Abstract Objective: To investigate effect of selfmade Tiaoqi Decoction on cisplatin induced renal injury in rats and its potential mechanism. Methods: Ninety male SpragueDawley rats were randomly divided into normal control group, model group, low, medium and high dose groups of Tiaoqi Decoction, and positive control group （n=15 for each group）. Cisplatin （2.5 mg/kg） was injected via the rat′s tail vein to induce kidney injury. The low, medium and high dose groups of Tiaoqi Decoction were administrated to rats at the doses of 2, 4, and 8 mL/kg by gavage. The positive control group was intraperitoneally injected with amifostine at a dose of 1 mg/kg, and the control group and model group rats were administrated with the normal saline （2 mL/kg） by gavage once a day for 60 consecutive days. The contents of serum urea nitrogen and creatinine were determined by colorimetry. The contents of tumor necrosis factorα （TNFα）, IL-6 and IL-1β of the kidney tissue, and the contents of TNF-α, IL-17 and matrix metalloproteinase-9 （MMP-9） secreted by spleen lymphocytes were determined by ELISA. The activity of superoxide dismutase （SOD） and the level of malondialdehyde were determined by colorimetric method and colorimetric method, respectively. Pathological changes of rat kidney tissue were examined by HE staining. The protein levels of Tolllike receptor 4 （TLR4） and NFκB in rat kidney tissue were tested by Western blotting. Results: Compared with the model group, urea nitrogen and creatinine levels of the serum, TNF-α, IL-6, IL-1β and MDA levels, TLR4 and NFκB protein levels of the kidney tissue, TNF-α, IL-17, MMP-9 levels of the spleen were significantly decreased in the low, medium and high dose groups of Tiaoqi Decoction （P<0.05）, but SOD activity was significantly increased（P<0.05）. The pathological changes of the kidney tissue was significantly attenuated. With the dose of Tiaoqi Decoction increasing, dosedependent changes of the indicators above were observed. Conclusion: Selfmade Tiaoqi Decoction could significantly attenuate renal injury induced by cisplatin in rats by inhibiting TLR4/NFκB pathway.

Received: 09 November 2020

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	Articles by authors
	LI Xiaolan1
	WANG Shaojun1
	MA Yuefei1
	WANG Linlin2

Cite this article:

LI Xiaolan1,WANG Shaojun1,MA Yuefei1等.

Effect of self-made Tiaoqi Decoction on cisplatin induced renal injury in rats and its mechanism

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 396-400.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I05/396

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