LncRNA44372在卵巢癌中的差异表达及生物信息学分析

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Abstract Objective: To explore the expression of lncRNA44372 and its potential mechanism in the development of ovarian cancer. Methods: The gene chip data GSE119054 of ovarian cancer samples and their control samples was extracted and downloaded from the NCBI GEO database, and the differential expressions of lncRNA in ovarian cancer were analyzed by using the CRN online tool. Annolnc was used to pick out the coexpression genes related to lncRNA44372. Gene Ontology and signal pathway enrichment analysis were performed to draw the outline of the possible biological function of lncRNA44372 in ovarian cancer by the online software David. The divinable ceRNA network of lncRNA44372 was constructed by the starbase, an online analysis tool. The expression of lncRNA44372 was detected by qRT-PCR in ovarian cancer cell lines. Results: In the GSE119054 data set, there were 6 upregulated lncRNA and 4 downregulated lncRNA in ovarian cancer. The related coexpressed genes of lncRNA44372 were enriched in some biological processes, such as cell apoptosis, cell cycle regulation, protein threonine/tyrosine phosphatase activity. The related co-expressed genes of lncRNA44372 were mostly involved in signaling pathways about cell apoptosis and tumor pathogenesis and progression. LncRNA44372 may act as a microRNA sponge to bind with miR190 and miR193 and regulate the expression of ULK2 and LONP2 genes in ovarian cancer. qRT-PCR showed that the expression of lncRNA44372 in ovarian cancer cells was lower than that in normal ovarian cells（P<0.05）. Conclusion: LncRNA44372 was lowly expressed in ovarian cancer and it may play a role in the development and progression of ovarian cancer by influencing cell apoptosis, cell cycle and so on.

Received: 02 June 2020

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	Articles by authors
	DING Jing1
	YANG Xinxin1
	ZOU Xueqin1
	GAO Shujun1
	LIU Xue1
	PAN Lingli1
	ZHANG Qiudan1
	ZHAO Yangjing1
	LIANG Xiuting2
	WANG Hui1
	ZHU Yanling2
	SHAO Qixiang1

Cite this article:

DING Jing1,YANG Xinxin1,ZOU Xueqin1等.

The differential expression and bioinformatics analysis of lncRNA44372 in ovarian cancer

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 391-395.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I05/391

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