miR-376c-3p通过靶向谷氨酸受体相互作用蛋白1影响乳腺癌细胞的增殖和迁移

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Abstract Objective: To investigate the expression level, biological function and molecular regulation mechanism of miR-376c3p in breast cancer cells.Methods: The expression level of miR-376c-3p in breast epithelial cell MCF-10A and breast cancer cells was detected by qRT-PCR, and then breast cancer cells MCF7 and MD-MB-231 were transfected with miR-376c-3p mimic.The proliferation was detected by colony formation assay.The migration and invasion were detected by Transwell assay and wound healing assay.Starbase database was used to predict the downstream target gene of miR-376c-3p, and the targeting relationship was verified by Western blotting.Results: Compared with human breast epithelial cell MCF-10A, the expression of miR-376C-3p was significantly down-regulated in breast cancer cells MCF-7 and MDA-MB-231（P<0.01）.Colony formation assay showed that overexpression of miR-376c-3p could inhibit the proliferation of breast cancer cells（all P<0.05）.Transwell assay and wound healing assay showed that the upregulation of miR-376c-3p inhibited the migration of breast cancer cells. Bioinformatics results showed that miR-376c-3p could bind to glutamate receptor interacting protein 1（GRIP1）, and Western blotting assay showed that miR-376c-3p could inhibit the expression of GRIP1 and vimentin, and increase E-cadherin expression in breast cancer cells.Conclusion: The expression of miR-376c-3p was down-regulated in breast cancer cells；overexpression of miR-376c-3p significantly inhibited the proliferation and migration ability of breast cancer cells in vitro, and miR376c3p may regulate the epithelialmesenchymal transition process of breast cancer by targeting GRIP1.

Received: 03 March 2021

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	Articles by authors
	WANG Chao
	GAO Yuting
	LIU Ziyao
	LI Tao
	WANG Jingzhe
	LIU Lu
	XIE Yan
	SUN Xiaochun

Cite this article:

WANG Chao,GAO Yuting,LIU Ziyao等.

miR-376c-3p regulates the proliferation and migration of breast cancer cells via targeting glutamate receptor interacting protein 1

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(05): 380-385.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I05/380

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