肿瘤成纤维细胞通过外泌体miR- 21促进膀胱癌细胞侵袭转移

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Abstract Objective: To explore the role and potential mechanism of exosomal involved in process of cancerassociated fibroblast （CAF） promoting the invasion and metastasis of bladder cancer cells. Methods: CAF and normal fibroblast （NF） was isolated from bladder cancer and normal tissues adjacent to the cancer, respectively. The expression of was assessed in tissues and cell lines of bladder cancer, CAF, NF and exosomes. The transwell invasion and metastasis assays were applied to test the effect of CAF, CAF induced exosomes and on the invasion and metastasis ability of bladder cancer cells. The confirmation of target gene of was performed with luciferase assay. Results: Compared with controls, was highly expressed in bladder cancer tissues, especially those with late stages. Exosomes released from CAF showed higher expression of than that from NF. Incubation with CAF, CAF secreted exosomes, or transfection of mimics could significantly enhance the ability of T24 cells to invade and migrate. Exosomal absorbed by bladder cancer cells could promote the invasion and metastasis by targeting PTEN. Conclusion: Exosomal could regulate the process of CAF promoting the invasion and metastasis of bladder cancer cells.

Received: 06 November 2020

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	Articles by authors
	WEI Yong1
	2
	CHEN Peng1
	ZHU Hongru1
	CHEN Quanbing1
	JIANG Yazhi1
	QIAN Wenhui1

Cite this article:

WEI Yong1,2,CHEN Peng1等.

Exosomal miR-21 secreted by CAF promotes invasion and metastasis of bladder cancer cells

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(04): 321-325，332.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I04/321

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