NFATc1对糖尿病动脉粥样硬化ApoE-/-小鼠肠道菌群的影响

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Abstract Objective: To explore the effect of nuclear factor of activated Tcell cytoplasmic 1（NFATc1）on the intestinal microflora of diabetic atherosclerosis ApoE-/- mice. Methods: ApoE-/- mice were used to make diabetic atherosclerosis models by the injection of Streptozotocin. AdenoAssociated Viral Vector was injected into the tail vein to interfere with the expression of NFATc1 in vivo. The mice were sacrificed 8 weeks later, and aorta tissues were separated for Western blotting to verify efficacy of the knockdown of NFATc1 in the aorta. The relative area of atherosclerotic plaque was analyzed by HE staining. Mice feces were collected and nuclear acid were extracted for detecting gene sequence by Illumina MiSeq. Class levels of intestinal microflora of mice were determined through bioinformatics analysis. Results: The mouse models of diabetic atherosclerosis were successfully made. After the NFATc1 knockdown AdenoAssociated Viral Vector was injected into the tail vein, its expression was significantly reduced in the aorta（P＜0.05）.HE staining showed that the formation of atherosclerotic plaques in the aorta was significant, but the relative area of atherosclerotic plaques decreased significantly after interfering with the expression of NFATc1, compared to the negative control group（P<0.05）. The 16S rRNA gene sequencing results showed that the relative abundance of Erysipelotrichi in the intestine of NFATc1 knockdown mice was significantly higher than that of negative control mice（78.82% vs. 32.93%，W=2）. Conclusion: NFATc1 could change the relative abundance of intestinal microflora in diabetic atherosclerosis ApoE-/- mice.

Received: 16 March 2021

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	Articles by authors
	LIU Jia
	SUN Zhen
	HOU Lina
	QIAN Yongjiang
	SHAO Chen
	YUAN Wei
	WANG Zhongqun

Cite this article:

LIU Jia,SUN Zhen,HOU Lina等. Effects of NFATc1 on intestinal microflora of diabetic atherosclerosis ApoE-/- mice[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(04): 314-320.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I04/314

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