N-乙酰半胱氨酸对哮喘小鼠模型气道炎症和氧化应激的影响

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Abstract Objective: To investigate the effect of N-acetylcysteine (NAC) on airway inflammation, oxidative stress in asthma model mice and its potential mechanism. Methods: Eighteen female BALB/c mice were randomly divided into control group, asthma group, and N-acetylcysteine(NAC) group, with 6 in each group. Asthma group and NAC group were atomized to construct asthma model, and NAC group was given NAC (300 mg/kg) by intragastric instillation 30 min before atomization, and control group was treated with the same volume of PBS. Samples were harvested within 24 h after the end of nebulization. Collection of bronchoalveolar lavage fluid was used to count inflammatory cells. The levels of serum cytokines IL-13, IL-5 and IFN-γ were measured by ELISA. HE staining was used to observe the pathological changes of mice lung tissue, and inflammation score was performed. The levels of malondialdehyde and glutathione(GSH) in lung tissue were measured by colorimetry. The expression of nuclear factor erythroid 2 related factor 2(Nrf2) and heme oxygenase1(HO-1) in lung tissue were measured by quantitative real-time PCR method. Results: Compared with the control group, there were lots of inflammatory cells infiltration in the lung tissue, the alveolar structure was obviously destroyed, the number of inflammatory cells, the levels of IL-5, IL-13, inflammation score, and the levels of malondialdehyde were significantly increased (all P<0.05), the levels of IFN-γ and GSH were significantly decreased(all P<0.05), the expression of Nrf2 mRNA and HO-1 mRNA showed no significant difference in asthma group. Compared with the asthma group, the infiltration of inflammatory cells in lung tissue and the destruction of alveolar structure were reduced, the number of inflammatory cells, IL-5, IL-13 contents, inflammation score, malondialdehyde contents were significantly decreased(all P<0.05), the contents of IFN-γ, GSH and the expression of Nrf2 mRNA and HO-1 mRNA were significantly increased in NAC group (all P<0.05). Conclusion: NAC could reduce airway inflammation and oxidative stress in asthmatic mice, which may be attributed to activation of Nrf2/HO-1 signaling pathway.

Received: 30 April 2020

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	Articles by authors
	XU Lili1
	ZHANG Jun2
	SHANG Lulu1
	DING Manling1
	ZHENG Jinxu1

Cite this article:

XU Lili1,ZHANG Jun2,SHANG Lulu1等.

Effects of N-acetylcysteine on airway inflammation and oxidative stress in asthma model mice

[J]. Journal of Jiangsu University(Medicine Edition), 2021, 31(01): 56-60.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2021/V31/I01/56

［1］Holgate ST, Wenzel S, Postma DS, et al. Asthma\[J\]. Nat Rev Dis Primers, 2015, 1(1): 15025.

［2］AlHarbi NO, Nadeem A, AlHarbi MM, et al. Oxidative airway inflammation leads to systemic and vascular oxidative stress in a murine model of allergic asthma\[J\]. Int Immunopharmacol, 2015, 26(1): 237-245.

［3］Mishra V, Banga J, Silveyra P. Oxidative stress and cellular pathways of asthma and inflammation:therapeutic strategies and pharmacological targets\[J\]. Pharmacol Ther, 2018, 181: 169-182.

［4］Sahiner UM, Birben E, Erzurum S, et al. Oxidative stress in asthma:part of the puzzle\[J\]. Pediatr Allergy Immunol, 2018, 29(9): 789-800.

［5］Loboda A, Damulewicz M, Pyza E, et al. Role of Nrf2/HO1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism\[J\]. Cell Mol Life Sci, 2016, 73(17): 3221-3247.

［6］Rangasamy T, Guo J, Mitzner WA, et al. Disruption of Nrf2 enhances susceptibility to severe airway inflammation and asthma in mice\[J\]. J Exp Med, 2005, 202(1): 47-59.

［7］Elbini Dhouib I, Jallouli M, Annabi A, et al. A minireview on Nacetylcysteine: An old drug with new approaches\[J\]. Life Sci, 2016, 151: 359-363.

［8］Cazzola M, Calzetta L, Page C, et al. Influence of Nacetylcysteine on chronic bronchitis or COPD exacerbations: a metaanalysis\[J\]. Eur Respir Rev, 2015, 24(137): 451-461.

［9］Zhang Y, Ding S, Li C, et al. Effects of Nacetylcysteine treatment in acute respiratory distress syndrome: a metaanalysis\[J\]. Exp Ther Med, 2017, 14(4): 2863-2868.

［10］Oldham JM, Witt LJ, Adegunsoye A, et al. Nacetylcysteine exposure is associated with improved survival in antinuclear antibody seropositive patients with usual interstitial pneumonia\[J\]. BMC Pulm Med, 2018, 18(1): 30.

［11］周冬梅,孔灵菲.N乙酰半胱氨酸对哮喘小鼠气道上皮细胞自噬水平的影响\[J\]. 中国医科大学学报,2018,47(7):626-630.

［12］Silveira JS, Antunes GL, Kaiber DB, et al. Reactive oxygen species are involved in eosinophil extracellular traps release and in airway inflammation in asthma\[J\]. J Cell Physiol, 2019, 234(1): 23633-23646.

［13］Eftekhari P, Hajizadeh S, Raoufy MR, et al. Preventive effect of Nacetylcysteine in a mouse model of steroid resistant acute exacerbation of asthma\[J\]. EXCLI J, 2013,12:184-192.

［14］Qin W, Deng T, Cui H, et al. Exposure to diisodecyl phthalate exacerbated Th2 and Th17mediated asthma through aggravating oxidative stress and the activation of p38 MAPK\[J\]. Food Chem Toxicol, 2018,114:78-87.

［15］Lee KS, Kim SR, Park HS, et al. A novel thiol compound, Nacetylcysteine amide, attenuates allergic airway disease by regulating activation of NFκB and hypoxiainducible factor1α\[J\]. Exp Mol Med, 2007, 39(6): 756-768.

［16］McBrien CN, MenziesGow A. The biology of eosinophils and their role in asthma\[J\]. Front Med (Lausanne), 2017, 4: 93.

［17］Radermecker C, Louis R, Bureau F, et al. Role of neutrophils in allergic asthma\[J\]. Curr Opin Immunol, 2018, 54: 28-34.

［18］Lambrecht BN, Hammad H, Fahy JV. The cytokines of asthma\[J\]. Immunity, 2019, 50(4):975-991.

［19］Fatani SH. Biomarkers of oxidative stress in acute and chronic bronchial asthma\[J\]. J Asthma, 2014, 51(6): 578-584.

［20］Fitzpatrick AM, Jones DP, Brown LA. Glutathione redox control of asthma: from molecular mechanisms to therapeutic opportunities\[J\]. Antioxid Redox Signal, 2012, 17(2): 375-408.

［21］Liu Q, Gao Y,Ci X. Role of Nrf2 and its activators in respiratory diseases\[J\]. Oxid Med Cell Longev, 2019, 2019: 7090534.

［22］Sussan TE, Gajghate S, Chatterjee S, et al. Nrf2 reduces allergic asthma in mice through enhanced airway epithelial cytoprotective function\[J\]. Am J Physiol Lung Cell Mol Physiol, 2015, 309(1): 27-36.