非编码 RNA GlmY 对伤寒沙门菌生物膜形成的影响

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Abstract Objective: To investigate the effect of non-coding RNA GlmY on the biofilm formation of Salmonella enterica serovar Typhi (S. Typhi) and its potential molecular mechanisms.Methods: The homologous recombination induced by the suicide plasmid pGMB151 was used to generate the glmY deleted mutant of S. Typhi. The bacterial biofilm formation in 96-microwell plate was detected by crystal violet staining. qRT-PCR was used to analyze the mRNA expression levels of biofilm formation-related genes in glmY deleted mutant and wild-type strain. Results: The glmY deletion mutant strain was successfully constructed. Compared with the wild-type strain, the glmY deletion mutant showed significantly weaker ability of biofilm formation. The qRT-PCR results showed that mRNA level of csgD and csgA was significantly decreased after deletion of GlmY. Conclusion: The non-coding RNA GlmY may enhance the biofilm formation of S. Typhi by up-regulating the expression of csgD, csgA and Curli synthesis.

Received: 23 February 2020

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	Articles by authors
	LI Xue1
	ZHAO Xin2
	JIN Meng-tong1
	TANG Hao1
	ZHANG Ying1
	HUANG Xin-xiang1

Cite this article:

LI Xue1,ZHAO Xin2,JIN Meng-tong1等.

Effect of non-coding RNA GlmY on biofilm formation of Salmonella enterica serovar Typhi

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(05): 435-438.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I05/435

［1］Larock DL, Chaudhary A, Miller SI. Salmonellae interactions with host processes\[J\]. Nat Rev Microbiol, 2015,13(4):191-205.

［2］Wain J, Hendriksen RS, Mikoleit ML, et al. Typhoid fever\[J\]. Lancet, 2015,385(9973):1136-1145.

［3］Hall CW, Mah TF. Molecular mechanisms of biofilmbased antibiotic resistance and tolerance in pathogenic bacteria\[J\]. FEMS Microbiol Rev, 2017,41(3):276-301.

［4］Wolska KI, Grudniak AM, Rudnicka Z, et al. Genetic control of bacterial biofilms\[J\]. J Appl Genet, 2016, 5(2): 225-238.

［5］Simm R, Ahmad I, Rhen M, et al. Regulation of biofilm formation in Salmonella enterica serovar Typhimurium\[J\]. Future Microbiol, 2014,9(11):1261-1282.

［6］Nitzan M, Rehani R, Margalit H. Integration of bacterial small RNAs in regulatory networks\[J\]. Annu Rev Biophys, 2017,46:131-148.

［7］孙嘉遥,陆仁飞,赵昕,等.小RNA SbfR增强伤寒沙门菌动力和侵袭力\[J\].江苏大学学报(医学版),2018,28(1):47-50.

［8］熊昌艳,李雪娇,黄新祥.伤寒沙门菌不同生长期和不同应激下非编码RNA ArpH的表达\[J\].江苏大学学报(医学版),2020,30(1):58-62.

［9］Khan MA, Gopel Y, Milewski S, et al. Two small RNAs conserved in enterobacteriaceae provide intrinsic resistance to antibiotics targeting the cell wall biosynthesis enzyme glucosamine6phosphate synthase\[J\]. Front Microbiol, 2016,7:908.

［10］汪伟伟, 夏歆, 王静瑜, 等. 伤寒沙门菌外膜孔道蛋白ompC、ompF缺陷株的制备及其耐药性\[J\]. 江苏大学学报(医学版), 2015,25(6):532-535.

［11］Zhao X, Liu R, Tang H, et al. A 3′UTRderived noncoding RNA RibS increases expression of cfa and promotes biofilm formation of Salmonella enterica serovar Typhi\[J\]. Res Microbiol, 2018,169(6):279-288.

［12］Reichenbach B, Maes A, Kalamorz F, et al. The small RNA GlmY acts upstream of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli\[J\]. Nucleic Acids Res, 2008,36(8):2570-2580.

［13］Kumar A, Alam A, Rani M, et al. Biofilms: Survival and defense strategy for pathogens\[J\]. Int J Med Microbiol, 2017,307(8):481-489.

［14］Newman SL, Will WR, Libby SJ, et al. The curli regulator CsgD mediates stationary phase countersilencing of csgBA in Salmonella Typhimurium\[J\]. Mol Microbiol, 2018,108(1):101-114.

［15］Smith DR, Price JE, Burby PE, et al. The production of curli amyloid fibers is deeply integrated into the biology of Escherichia coli\[J\]. Biomolecules, 2017,7(4):75.

［16］Jrgensen MG, Thomason MK, Havelund J, et al. Dual function of the McaS small RNA in controlling biofilm formation\[J\]. Genes Dev, 2013,27(10):1132-1145.

［17］Andreassen PR, Pettersen JS, Szczerba M, et al. sRNAdependent control of curli biosynthesis in Escherichia coli: McaS directs endonucleolytic cleavage of csgD mRNA\[J\]. Nucleic Acids Res, 2018,46(13):6746-6760.

［18］Holmqvist E, Reimegrd J, Sterk M, et al. Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis\[J\]. EMBO J, 2010,29(11):1840-1850.