人神经干细胞微囊泡对谷氨酸诱导PC12细胞损伤的保护作用

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Abstract Objective: To investigate the effect of human neural stem cell microvesicles (hNSCMVs) against glutamate-induced cytotoxicity in PC12 cells and its possible mechanism. Methods: Excitative injury model was established by treating rat adrenal medulla pheochromocytoma PC12 cells with glutamate. Experiment was divided into three groups: control group, PC12 cells without any treatment; glutamate group, PC12 cells treated with glutamate for 24 h; hNSC-MVs + glutamate group, PC12 cells were pretreated with 200 mg/L hNSC-MVs for 24 h, and then treated with glutamate for 24 h. MTT method was used to detect the survival rate of PC12 cells and Western blotting was used to determine the expression of apoptosis-related proteins, Bax and Bcl-2. Results: PC12 cells could internalize hNSC-MVs. Glutamate has a toxic effect on PC12 cells, and its half inhibitory concentration is 25 mmol/L. Compared with glutamate group, the survival rate of PC12 cells in hNSC-MVs + glutamate group was surprisingly increased(P<0.01). Compared with control group, the expression of Bax protein in glutamate group was markedly increased, and the expression of Bcl-2 protein was greatly reduced. Compared with glutamate group, the expression of Bax protein in hNSC-MVs + glutamate group was significantly down-regulated, and the expression of Bcl-2 protein was remarkably up-regulated(all P<0.01). Conclusion: hNSC-MVs may play a protective role by inhibiting glutamate-induced PC12 cell apoptosis.

Received: 27 February 2020

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	Articles by authors
	YU Jia-hong
	TONG Yu
	YE Kai
	CHEN Tian-yan
	TANG Bin
	HU Jia-bo

Cite this article:

YU Jia-hong,TONG Yu,YE Kai等.

Protective effect of human neural stem cell microvesicles against glutamateinduced cytotoxicity in PC12 cells

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 302-306.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I04/302

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