脐带间充质干细胞外泌体对大鼠肾间质纤维化的抑制作用

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Abstract Objective: To investigate the repair effect of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Ex) on renal tubular interstitial fibrosis induced by unilateral ureteral obstruction (UUO) in rats. Methods: Human umbilical cord-derived mesenchymal stem cells were isolated and cultured, and hucMSC-Ex was extracted from the culture supernatant by ultrafiltration and gradient centrifugation. A unilateral ureteral ligation method was used to construct a renal interstitial fibrosis model. All rats were randomly divided into sham operation group， UUO group and hucMSC-Ex treatment group. Two hundred-fifty μg hucMSC-Ex was injected into the tail vein after the operation of treatment group rats. Seven days later, kidney tissues were collected and the general appearance of the kidneys in each group was observed. Western blotting was used to detect the expression of protein related to renal fibrosis: TGF-β1, E-cadherin and Vimentin. The kidney tissue sections were stained with HE staining, Masson staining and immunohistochemical staining to measure rat renal pathological changes, inflammatory cell infiltration and the degree of renal fibrosis in the obstructed rats. Results: The inflammatory cell infiltration in the kidney of UUO group significantly increased, the tissue structure was severely damaged, and extensive collagen deposition was observed. After hucMSC-Ex treatment, the tissue morphology and structure of were partially recovered, collagen deposition and inflammatory cell infiltration were reduced. At the same time, the expression of α-SMA, TGF-β1 and Vimentin in the kidney of UUO group increased, while the expression of E-cadherin decreased. However, hucMSC-Ex treatment could inhibit the expression of α-SMA and TGF-β1 and reverse the epithelial-mesenchymal conversion. Conclusion: hucMSC-Ex can reduce the pathological damage of renal tissue in rats and delay the progress of renal interstitial fibrosis in rats.

Received: 05 February 2020

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	Articles by authors
	HU Yu-yan
	YIN Lei
	ZHANG Chen-xiao
	QIAN Hui

Cite this article:

HU Yu-yan,YIN Lei,ZHANG Chen-xiao等.

The inhibition of umbilical cord mesenchymal stem cell-derived exosomes on rat renal interstitial fibrosis

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(04): 282-286.

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I04/282

［1］Humphreys BD. Mechanisms of renal fibrosis\[J\]. Ann Rev Physiol, 2016, 80(1): 1-18.

［2］Liu F, Zhuang S. New therapies for the treatment of renal fibrosis\[J\]. Adv Exp Med Biol, 2019, 1165: 625-659.

［3］Pegtel DM, Gould SJ. Exosomes\[J\]. Annu Rev Biochem, 2019, 88: 487-514.

［4］Jiang WQ, Tan YW, Cai MJ, et al. Human umbilical cord MSCderived exosomes suppress the development of CCl4induced liver injury through antioxidant effect\[J\]. Stem Cells Int, 2018，2018: 6079642.

［5］Mao F, Wu YB, Tan XD, et al. Exosomes derived from human umbilical cord mesenchymal stem cells relieve inflammatory bowel disease in mice\[J\]. Biomed Res Int, 2017, 2017: 5356760.

［6］Zhang B, Wu X, Zhang X, et al. Human umbilical cord mesenchymal stem cell exosomes enhance angiogenesis through the Wnt4/βcatenin pathway\[J\]. Stem Cells Transl Med, 2015, 4(5): 513-522.

［7］Wang BY, Jia HY, Zhang B, et al. Preincubation with hucMSCexosomes prevents cisplatininduced nephrotoxicity by activating autophagy\[J\]. Stem Cell Res Ther, 2017, 8(1): 75.

［8］俞静, 张荣雪, 贾浩源, 等. 脐带间质干细胞来源的外泌体抑制TGFβ1诱导的NRK52E细胞上皮间质转化\[J\]. 江苏大学学报（医学版）, 2017,27（4）: 277-280.

［9］Jia HY, Liu WZ, Zhang B, et al. Enhanced autophagy via modulation of ATG16L in preventing cisplatininduced acute kidney injury\[J\]. Am J Transl Res, 2018, 10(1): 101-113.

［10］Hosseini SF, Alibolandi M, Rafatpanah H, et al. Immunomodulatory properties of MSCderived exosomes armed with high affinity aptamer toward mylein as a platform for reducing multiple sclerosis clinical score\[J\]. J Control Release, 2019, 299: 149-164.

［11］Ralf W, Sabine W, Frank T. Organ and tissue fibrosis: molecular signals, cellular mechanisms and translational implications\[J\]. Mol Aspects Med, 2019, 65: 2-15.

［12］Meng XM, Nikolic PD, Lan HY. TGFβ: the master regulator of fibrosis\[J\]. Nat Rev Nephrol, 2016, 12(6): 325-338.

［13］Yao Y, Chen R, Wang G, et al. Exosomes derived from mesenchymal stem cells reverse EMT via TGFβ1/Smad pathway and promote repair of damaged endometrium\[J\]. Stem Cell Res Ther, 2019, 10(1): 225.

［14］Nieto MA, Huang RY, Jackson RA, et al. EMT: 2016\[J\]. Cell, 2016, 166(1): 21-45.

［15］Li T, Yan Y, Wang B, et al. Exosomes derived from human umbilical cord mesenchymal stem cells alleviate liver fibrosis\[J\]. Stem Cells Dev, 2013, 22(6): 845-854.

［16］Lai RC, Yeo RW, Lim SK. Mesenchymal stem cell exosomes \[J\]. Semin Cell Dev Bio, 2015, 40: 82-88.

［17］Wang B, Yao K, Huuskes BM, et al. Mesenchymal stem cells deliver exogenous microRNAlet7c via exosomes to attenuate renal fibrosis\[J\]. Mol Ther, 2016, 24(7): 1290-1301.

［18］Wang H, Wang B, Zhang A, et al. Exosomemediated miR29 transfer reduces musatrophy and kidney fibrosis in mice\[J\]. Mol Ther, 2019, 27(3): 571-583.