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The sensitization effect of RNAi silenced Sox2 expression on 5-FU in colorectal cancer cells
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| YUN Ya-jing1, XU Li-Xiao2, SUN Bin1, FENG Xing1 |
(1. Department of Neonatology, 2. Institute of Pediatrics，Children′s Hospital of Soochow University, Suzhou Jiangsu 215000, China) |
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Abstract [Abstract]Objective: To investigate the relationship between Sox2 and 5fluorouracil (5FU) induced chemotherapy sensitivity of human colon cancer cells (SW620) and gene expression in drugresistant families,and to explore the mechanism of chemotherapy resistance in colorectal cancer cells. Methods: After silencing SW620 cells with lentiviral packaging interference plasmid Sox2, Western blot, RTPCR and immunofluorescence staining were used to detect the changes in the expression of Sox2 in cells before and after interference,CCK8 assay was used to detect the sensitivity of cells to 5FU,Flow cytometry was used to detect the apoptosis rate of cells and the expression of drugresistant family genes. Results: After targeting Sox2 interference, the expression of Sox2 mRNA and protein was downregulated, and the positive expression rate of Sox2 was significantly reduced. After interfering with Sox2, the sensitivity of SW620 cells to 5FU was higher than that of control cells,the IC50 value decreased significantly,and there was a significant statistical difference between cells (P<0.05).The apoptosis rate of cells after Sox2 interference was significantly higher than that of control cells,and the expression level of ABC drugresistant family genes decreased significantly after Sox2 interference. Conclusion: After interfering with Sox2, the expression of Sox2 in SW620 cells was effectively inhibited. After interfering with Sox2, the sensitivity of SW620 cells to 5FU was significantly improved,and the expression level of ABC drugresistant genes was decreased, providing the possibility for seeking new gene targeted therapy.
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Received: 30 November 2019
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