FLCN在子宫颈鳞癌组织中的表达及其对子宫颈鳞癌SiHa细胞增殖及凋亡的影响

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Abstract [Abstract]Objective: To investigate the expression of folliculin(FLCN) in cervical squamous cancer tissue and its effect on the cell proliferation and apoptosis in cervical carcinoma. Methods: The mRNA and protein expressions of FLCN in cervical squamous cancer tissue were detected by qRTPCR and immunohistochemical technique. After overexpression or knocking down of FLCN in cervical squamous cancer SiHa cells, the FLCN mRNA expression was detected by qRT-PCR, the cell proliferation was evaluated by CCK8 -and phosphorylated histone H3 (PH3) staining, the cell apoptosis was measured by TUNEL. Results: Compared with the control group, FLCN mRNA and protein level expression in cervical squamous cancer groups were significantly reduced (P<0.05). After FLCN siRNA was transfected, cell proliferation increased and apoptosis decreased, PH3 protein positive ratio increased, TUNEL positive cell ratio decreased (P<0.05 or <0.01 ). Afer overexpression of FLCN, cell proliferation was inhibited and apoptosis was enhanced, the proportion of PH3 proteinpositive cells decreased, and the proportion of TUNELp-ositive cells increased(all P<0.01). Conclusion: FLCN gene was lowexpression in cervical squamous cancer tissue, which could inhibit proliferation and promote apoptosis of SiHa cells.

Received: 30 October 2019

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	Articles by authors
	ZHANG Yu
	YANG Pei-fang
	CHEN Xia
	LUAN Xiao-jin
	YAN Yi-dan
	YU Jun

Cite this article:

ZHANG Yu,YANG Pei-fang,CHEN Xia等.

Expression of FLCN in cervical squamous cancer tissue and its effect on proliferation and apoptosis in SiHa cells

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(01): 63-67.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I01/63

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