敲减lincRNA-p21的巨噬细胞对结肠癌细胞CT26生物学功能的影响

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Abstract [Abstract\]Objective: To investigate the effect of differential expression of lincRNAp21 in macrophage on the tumor growth and tumorbearing mice, and provide a new target for the diagnosis and treatment of cancers. Methods: CT26 tumor cells were cultured and supernatant were collected to stimulate mouse macrophages RAW264.7 . The expression of IL-6 ,IL-4, IL-10, lincRNA-p21 were detected by RTqPCR. The expression of lincRNAp21 in macrophages transfected with si-lincRNA-p21 was detected by RT-qPCR. Co-cultured with CT26 after the macrophages transfected with NC and silincRNAp21, the apoptosis of CT26 was detected by flow cytometry, and the migration ability of CT26 was detected by transwell and wound healing assay. CT26 colon cancer tumor-bearing mice model were established, macrophages transfected with NC and si-lincRNA-p21 were injected to measure the volume and weight of tumor in tumor-bearing mice. Results: The expression of IL-6 was down-regulated (P<0.01) but the expression of IL4 and IL10 were upregulated (P<0.01) compared with the normal RAW264.7. LincRNA-p21 expression in RAW264.7 was higher than that in normal RAW264.7 (P<0.01). Compared with the NC group, the expression of lincRNA-p21 in macrophages transfected with si-lincRNA-p21 was down-regulated (P<0.01). Co-cultured with CT26 after the macrophages transfected with NC and si-lincRNA-p21, the expression of IL-6 was up-regulated (P<0.01) but the expression of IL4 and IL10 were downregulated (P<0.01). Compared with the NC group, the apoptosis of CT26 cells co-cultured with the macrophages transfected with si-lincRNAp21 group were increased (P<0.01), and the migration ability decreased (P<0.05). Compared with the NC group, the tumor volume and weight of tumor in tumorbearing mice injected with silincRNA-p21 macrophages were decreased (P<0.05) . Conclusion: The knockdown of lincRNA-p21 in macrophage can promote the increase of apoptosis of CT26 and inhibit its ability of migration and delay the growth of tumor in tumorbearing mice.

Received: 09 June 2019

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	Articles by authors
	ZHOU Li-ning1
	2
	LIU Yue-qin3
	SU Zhao-liang1
	ZHOU Feng2
	XU Hua-xi1

Cite this article:

ZHOU Li-ning1,2,LIU Yue-qin3等.

Effect of lincRNA-p21 knockdown in macrophage on the biological function of colon cancer cells CT26

[J]. Journal of Jiangsu University(Medicine Edition), 2020, 30(01): 12-17.

URL:

http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2020/V30/I01/12

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