人参炔醇重塑巨噬细胞表型调控乳腺癌细胞生物学行为

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Abstract Objective: To investigate the effects of panaxynol on the phenotype and function of macrophages and the biological behavior of breast cancer cells. Methods: Firstly, breast cancer 4T1 cells were directly treated with panaxynol, and various biological behavior changes were observed. Secondly, the macrophage cell line RAW264.7 was stimulated with breast cancer cell 4T1 culture supernatant for 48 h to induce macrophage polarization to M2 macrophages, and then macrophages were treated with different concentrations of panaxynol(0, 8, 16 μg/mL) for 24 h. qRTPCR, Western blotting, flow cytometry and ELISA were used to detect the phenotypic and antigenpresenting related molecules expression changes, such as MHCⅡ, CD80 and CD86, and the secretion spectrum changes, and the influence of panaxynol on the migration and invasion of breast cancer cell lines. Results: Compared with the 0 μg/mL group, the breast cancer cells proliferation was inhibited by the treatment with 8, 16 μg/mL panaxynol(P＜0.01), the cell apoptosis rate was enhanced(P＜0.05), and the chemotaxis of cancer cells was weakened. In addition, panaxynolstimulated macrophage showed the M2 phenotype. After stimulation with panaxynol(16 μg/mL), the macrophage phenotype was reversed, the expression of Arg1 was decreased(P＜0.01), while the expression of iNOS was increased(P＜0.01), and the surface antigenpresenting molecules(MHCⅡ, CD80, CD86) was increased significantly, accompanied by the expression level of IL4, IL8, IL10 downregulated(P＜0.01) and IL1β, TNFα upregulated(all P＜0.05). In addition, panaxynolstimulated macrophages could significantly reduce the number of migrating and invading breast cancer cells. Conclusion: Panaxynol could remodel macrophage phenotype and function, and therefore regulate the biological behavior of breast cancer cells.

Received: 04 March 2019

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http://zzs.ujs.edu.cn/xbyxb/EN/ OR http://zzs.ujs.edu.cn/xbyxb/EN/Y2019/V29/I04/287

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