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| Microarray and bioinformatics analyses reveal association of microRNA-125b-5p with gastric cancer
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| ZHANG Xuan-feng 1,2, CHEN Zheng-wei 1,2， ZHANG An-wei 1,2, BU Xue-feng 2 |
| （1. School of Medicine, Jiangsu University, Zhenjiang Jiangsu 212013; 2. Department of General Surgery, Affiliated People′s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002,China)
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Abstract Objective: To analyze the expression of microRNA in gastric cancer by bioinformatics. Methods: The differential expression of microRNAs in two subsets of GEO dataset was analyzed. Using R software package. The expressions of microRNAs in 60 gastric cancer surgical specimens and 20 paracancerous surgical specimens were detected by qRT-PCR. The relationships between microRNA expression and differentiation, TNM stage, sex, age, invasion, family history and tumor diameter were analyzed. Results: The expression of miRNA-125b-5p was up-regulated in GSE93415 and GSE99415, and the difference was statistically significant(P<0.05). The similar expression trend was confirmed in gastric cancer and normal gastric tissues with qRT-PCR. The expression of miRNA-125b-5p was correlated with TNM stage and invasion degree(P<0.05), but not with age, sex, family history, tumor diameter and differentiation degree(P>0.05).The survival time of gastric cancer patients with high expression of miR-125b-5p was significantly reduced. Conclusion: miR-125b-5p is associated with TNM stage, invasion and survival time of gastric cancer.
［Key words］miR-125b-5p; gastric cancer; bioinformatics; GEO microarray datasets
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Received: 20 December 2018
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2019, Vol. 29 
