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| Feasibility of evaluation of radiationinduced soft tissue injury
by multimouse MRI |
| XIONG Bowen1, XING Zhen2, WANG Zeng3,4, KANG Nannan1, PAN Xiaoxian1,
CHEN Xiuying5, SU XIONG Bo-wen1, XING Zhen2, WANG Zeng3,4, KANG Nan-nan1, PAN Xiao-xian1,CHEN Xiu-ying5, SU Li4,5, HONG Jin-sheng4,5 |
| （1. The First Clinical College，Fujian Medical University，Fuzhou Fujian 350005; 2. Department of Radiology,the First Affiliated Hospital of Fujian Medical University,Fuzhou Fujian 350005; 3. Central Laboratory, the First Affiliated Hospital of Fujian Medical University,Fuzhou Fujian 350005; 4. Key Laboratory of Radiation Biology, Fujian Higher Education, Fuzhou Fujian 350005; 5. Department of Radiotherapy, the First Affiliated Hospital of Fujian Medical University,Fuzhou Fujian 350005, China）
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Abstract Objective: To investigate the feasibility of evaluation of radiationinduced soft tissue injuries in multiple mice by application of 3.0T magnetic resonance imaging(MRI). Methods: Twenty eightweekold male C57BL/6 mice were randomly divided into control group and irradiation group, 10 mice in each group.The right hind limb of mouse in the irradiation group was irradiated 30 Gy with 6 MV Xray in a single shot and the animals in the control group was irradiated with sham. 3.0T MRI examination was performed before irradiation and the 7th day after irradiation,and 5-6 mice were simultaneously examined at a time. The differences of the apparent diffusion coefficient(ADC) value and the longitudinal relaxation time(T1) value of the right hind thigh muscles between the two groups of mice were compared. Results: MRI images of hind limbs in mice were of good quality and high contrast. There was no significant difference in ADC value and T1 value of the right hind thigh muscles between the two groups before irradiation(P>0.05). There was also no significant difference in ADC value and T1 value of the thigh muscles between the two hind limbs of the irradiated group mice on the 7th day after irradiation(P>0.05). On the 7th day after irradiation, the ADC values of the right hind thigh muscle of the control group and the irradiated group were (1.34±0.09)×10-3mm2/s and (1.45±0.11)×10-3mm2/s, respectively. The later was significantly higher than the former(t=2.473,P=0.023). In addition, the T1 values of the radiated group［(1 514.9±110.5) ms］ was significantly higher than those of the control group［(1 394.9±70.8) ms, t=2.890, P=0.010］. Conclusion: It is a simple and feasible method to use 3.0T MRI to simultaneously examine multiple mice for evaluating radiationinduced soft tissue injuries in mice.
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Received: 09 November 2018
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| 1］Straub JM, New J, Hamilton CD, et al. Radiationinduced fibrosis: mechanisms and implications for therapy\[J\]. J Cancer Res Clin Oncol, 2015,141(11):1985-1994.［2］Kurow O,Frey B, Schuster L,et al. Full length interleukin 33 aggravates radiationinduced skin reaction\[J\]. Front Immunol, 2017,8:722.［3］Shuyu Z, Wenjie W, Qing G, et al. Protein and miRNA profiling of radiationinduced skin injury in rats: the protective role of peroxiredoxin6 against ionizing radiation\[J\]. Free Radic Biol Med, 2014,69(4):96-107.［4］Xiao Z, Yang S, Su Y, et al. Alteration of the inflammatory molecule network after irradiation of soft tissue\[J\].Adv Exp Med Biol, 2013,765:335-341.［5］Cui P, Feng X, Zhu F, et al. Acute respiratory distress syndrome induced by White Smoke Inhalation: a potential animal model for evaluating pathological changes and underlying mechanisms\[J\]. Cell Physiol Biochem, 2018,47(6):2396-2406.［6］Baudouy D, Michiels JF, Vukolic A, et al. Echocardiographic and histological examination of cardiac morphology in the mouse\[J\]. J Vis Exp, 2017(128).doi: 10.3791/55843.［7］Chaney AM, Johnson EM, Cropper HC, et al. PET imaging of neuroinflammation using \[11C\]DPA713 in a mouse model of ischemic stroke\[J\]. J Vis Exp, 2018(136).doi: 10.3791/57243.［8］Chp J,Reimann C,Brangsch J, et al. In vivo MRangiography for the assessment of aortic aneurysms in an experimental mouse model on a clinical MRI scanner: Comparison with highfrequency ultrasound and histology\[J\]. PLoS One, 2017,12(6):e0178682.［9］Brown SD, Nolan PM. Mouse mutagenesissystematic studies of mammalian gene function\[J\]. Hum Mol Genet, 1998,7(10):1627-1633.［10］Bock NA,Konyer NB,Henkelman RM.Multiplemouse MRI\[J\].Magn Reson Med,2003,49(1): 158-167.［11］Abdel Razek AAK. Routine and advanced diffusion imaging modules of the salivary glands\[J\]. Neuroimaging Clin N Am, 2018,28(2):245-254.［12］Kauppinen RA. Monitoring cytotoxic tumour treatment response by diffusion magnetic resonance imaging and proton spectroscopy\[J\]. NMR Biomed, 2002,15(1):6-17.［13］祁丽, 张龙江, 卢光明. 心肌磁共振T1 Mapping技术及其临床应用进展\[J\]. 医学研究生学报, 2015,28(6):650-655.［14］Hueper K, Peperhove M, Rong S, et al. T1mapping for assessment of ischemiainduced acute kidney injury and prediction of chronic kidney disease in mice\[J\]. Eur Radiol, 2014,24(9):2252-2260.［15］Bock NA, Nieman BJ, Bishop JB, et al. In vivo multiplemouse MRI at 7 Tesla\[J\]. Magn Reson Med, 2005,54(5):1311-1316.［16］Ramirez MS, EsparzaCoss E, Bankson JA. Multiplemouse MRI with multiple arrays of receive coils\[J\]. Magn Reson Med, 2010,63(3):803-810. |
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2019, Vol. 29 
