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Pharmacokinetic characteristics in rats of the active components in Erzhi Formula |
WANG Hua-jun1, JIANG Jun2, YIN Jiang-ning3 |
(1. Department of Pharmacy, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. School of Pharmacy, Jiangsu University, Zhenjiang Jiangsu 212013; 3. Department of Emergency, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China) |
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Abstract Objective: To elucidate the pharmacokinetic characteristics of terpenoids in Erzhi Formula in rats. Methods: The analytic technique combined with HPLC was carried out to determine the content of Oleanolic acid and Ursolic acid in rats′ plasma and tissue after being fed Erzhi Formula. Then the DAS 2.0 program was used to calculate their corresponding pharmacokinetic parameters and drew the plasma concentrationtime curve. Results: The regression equation of Oleanolic acid and Ursolic acid were as Y=6.510 975 36X+2.180 605 9(R2=0.999 78) and Y=5.412 280 24X-1.627 312 6(R2=0.999 18), respectively. Linear range was 0.5-50.0 μg/mL; LOD were as 1.24 and 1.26 μg/mL, respectively. The recovery rates of the Erzhi Formula were 99.8% and 102.1%; the extraction recoveries from plasma samples were as 82.15% and 83.73%, respectively. The content range of Oleanolic acid and Ursolic acid in 7 batches of Erzhi Formulas were 0.67%-4.26% and 0.21%-1.19%, respectively; the concentrations of Oleanolic acid in the liver, heart, spleen, lung and kidney tissues were sequentially 197.60,11.70,1.10,43.70 and 40.10 mg/L. The concentrations of Ursolic acid in the liver, heart, spleen, lung and kidney tissues were 92.90, 61.80, 37.90, 307.50 and 301.30 mg/L. Tmax of both Oleanolic acid and Ursolic acid were 0.5 h; Cmax of Oleanolic acid and Ursolic acid were as (111.33±10.50) mg/L and (153.72±3.35) mg/L, respectively. AUC (0-∞) of Oleanolic acid and Ursolic acid were as (342.44±10.15) mg/(L·h) and (589.79±9.49) mg/(L·h), respectively. T1/2z of Oleanolic acid and Ursolic acid were as (2.93±0.03) h and (3.19±0.02) h, respectively. Conclusion: The method can be used to detect the contents of terpenoids in Erzhi Formula, plasma and tissue.
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Received: 31 August 2017
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