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| A pilot study of serum microRNA profiles as a novel biomarker for primary biliary cirrhosis |
| PAN Teng-li1, 2, SUN Li2, ZHOU Xing-bei2, CHEN Li2, YU Xue-jun2, TAN You-wen2 |
| (1.School of Clinical Medicine, Jiangsu University, Zhenjiang Jiangsu 212001; 2.Department of Liver Diseases, the Third People′s Hospital of Zhenjiang, Zhenjiang Jiangsu 212005, China) |
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Abstract Objective: To screen serum microRNA profiles by high throughput sequencing technology method as diagnostic markers for primary biliary cirrhosis (PBC). Methods: First, we employed Illumina GA IIx deep sequencing for the initial screening to indicate the read numbers of microRNAs expression in serum pool of 3 PBC and 3 healthy controls, respectively. Second, qRT-PCR validation study was conducted in more samples. Finally, computer analysis was conducted to predict target genes and biological functions. Results: Twenty-two miRNAs, whose fold change were greater than 2, were filtered out by high throughput sequencing technology. After qRT-PCR validation, the serum expression levels of miR-122, miR-34a and miR-141 were significantly higher in PBC, consistent with the sequencing results. Target genes of prediction results showed that microRNAs played a role in signal transduction, in the nucleus and cytoplasm, through combined with protein. Conclusion: The distinguishing expressed microRNAs had certain reference value on the diagnosis of PBC.
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| [1] |
. [J]. Journal of Jiangsu University(Medicine Edition), 2018, 28(05): 454-458. |
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2014, Vol. 24 
